McConkey D J, Hartzell P, Duddy S K, Håkansson H, Orrenius S
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
Science. 1988 Oct 14;242(4876):256-9. doi: 10.1126/science.3262923.
Suspensions of thymocytes from young rats were incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), which resulted in a sustained increase in cytosolic free Ca2+ concentration followed by DNA fragmentation and loss of cell viability. Both the Ca2+ increase and DNA fragmentation were prevented in cells treated with the inhibitor of protein synthesis, cycloheximide, and DNA fragmentation and cell killing were not detected when cells were incubated in a "Ca2+-free" medium or pretreated with high concentrations of the calcium probe, quin-2 tetraacetoxymethyl ester. These results indicate that TCDD can kill immature thymocytes by initiating a suicide process similar to that previously described for glucocorticoid hormones.
将幼鼠胸腺细胞悬液与2,3,7,8-四氯二苯并对二恶英（TCDD）一起孵育，结果导致胞质游离Ca2+浓度持续升高，随后出现DNA片段化和细胞活力丧失。在用蛋白质合成抑制剂环己酰亚胺处理的细胞中，Ca2+升高和DNA片段化均受到抑制，并且当细胞在“无Ca2+”培养基中孵育或用高浓度的钙探针喹-2四乙酰氧基甲酯预处理时，未检测到DNA片段化和细胞死亡。这些结果表明，TCDD可通过启动类似于先前描述的糖皮质激素的自杀过程来杀死未成熟胸腺细胞。
