Karapetsas Athanasios, Voulgaridou Georgia-Persephoni, Iliadi Dimitra, Tsochantaridis Ilias, Michail Panagiota, Kynigopoulos Spyridon, Lambropoulou Maria, Stavropoulou Maria-Ioanna, Stathopoulou Konstantina, Karabournioti Sofia, Aligiannis Nektarios, Gardikis Konstantinos, Galanis Alex, Panayiotidis Mihalis I, Pappa Aglaia
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
Laboratory of Histology and Embryology, School of Medicine, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece.
Antioxidants (Basel). 2020 Jun 30;9(7):566. doi: 10.3390/antiox9070566.
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.
在本研究中，我们旨在检测不同植物来源和地理来源的希腊蜂蜜样品的抗氧化、抗衰老和光保护特性。使用乙酸乙酯提取物，并评估其总酚/黄酮含量和抗氧化能力。然后使用人永生化角质形成细胞（HaCaT）和/或重组人皮肤组织模型研究蜂蜜提取物对紫外线B（UVB）诱导的光损伤的细胞保护特性。具体而言，检测了特定基质金属蛋白酶（MMPs）的细胞毒性、氧化状态、DNA损伤和基因表达水平。总体而言，用蜂蜜提取物处理HaCaT细胞可降低DNA链断裂水平，并减轻UVB照射后细胞活力的下降。此外，蜂蜜提取物显著降低了受照射细胞的总蛋白羰基含量，然而，它们对细胞的总抗氧化状态没有显著影响。最后，在重组皮肤组织模型中，提取物减轻了UVB诱导的MMPs - 3、- 7和- 9的上调。总之，蜂蜜提取物在UVB照射条件下表现出显著的光保护和抗衰老特性，因此可进一步开发为有前景的新型天然抗衰老化妆品原料。
