基于 ATP 结合盒蛋白的系统发育分析表明埃及伊蚊（双翅目：蚊科）中存在一个新的 ABC 蛋白亚家族 J。
Phylogenetic analysis of the ATP-binding cassette proteins suggests a new ABC protein subfamily J in Aedes aegypti (Diptera: Culicidae).
机构信息
Laboratório de Bioquímica e Biologia Molecular de Vetores, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, 21941-617, Brazil.
出版信息
BMC Genomics. 2020 Jul 6;21(1):463. doi: 10.1186/s12864-020-06873-8.
BACKGROUND
We performed an in-depth analysis of the ABC gene family in Aedes aegypti (Diptera: Culicidae), which is an important vector species of arthropod-borne viral infections such as chikungunya, dengue, and Zika. Despite its importance, previous studies of the Arthropod ABC family have not focused on this species. Reports of insecticide resistance among pests and vectors indicate that some of these ATP-dependent efflux pumps are involved in compound traffic and multidrug resistance phenotypes.
RESULTS
We identified 53 classic complete ABC proteins annotated in the A. aegypti genome. A phylogenetic analysis of Aedes aegypti ABC proteins was carried out to assign the novel proteins to the ABC subfamilies. We also determined 9 full-length sequences of DNA repair (MutS, RAD50) and structural maintenance of chromosome (SMC) proteins that contain the ABC signature.
CONCLUSIONS
After inclusion of the putative ABC proteins into the evolutionary tree of the gene family, we classified A. aegypti ABC proteins into the established subfamilies (A to H), but the phylogenetic positioning of MutS, RAD50 and SMC proteins among ABC subfamilies-as well as the highly supported grouping of RAD50 and SMC-prompted us to name a new J subfamily of A. aegypti ABC proteins.
背景
我们对埃及伊蚊（双翅目：库蚊科）的 ABC 基因家族进行了深入分析，埃及伊蚊是虫媒病毒感染（如基孔肯雅热、登革热和寨卡热）的重要媒介物种。尽管它很重要，但以前对节肢动物 ABC 家族的研究并未集中在这个物种上。害虫和媒介对杀虫剂的抗性报告表明，其中一些 ATP 依赖性外排泵参与化合物运输和多药耐药表型。
结果
我们在埃及伊蚊基因组中鉴定出 53 个经典完整 ABC 蛋白。对埃及伊蚊 ABC 蛋白进行了系统发育分析，将新蛋白分配到 ABC 亚科中。我们还确定了 9 个全长 DNA 修复（MutS、RAD50）和染色体结构维持（SMC）蛋白序列，这些蛋白包含 ABC 特征。
结论
将假定的 ABC 蛋白纳入基因家族的进化树后，我们将埃及伊蚊 ABC 蛋白分类为已建立的亚科（A 至 H），但 MutS、RAD50 和 SMC 蛋白在 ABC 亚科中的系统发育定位以及 RAD50 和 SMC 的高度支持分组促使我们为埃及伊蚊 ABC 蛋白命名了一个新的 J 亚科。
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