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中国黄精花叶相关病毒1的全基因组序列,马铃薯Y病毒属的一个新成员

Complete genome sequence of polygonatum mosaic-associated virus 1, a novel member of the genus Potyvirus in China.

作者信息

Li Qiannan, Zhang Boxin, Hu Jingyu, Zhang Lei, Ji Pengzhang, Dong Jiahong

机构信息

School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource, Yunnan University of Chinese Medicine, Yunnan, 650500, China.

Institute of Medicinal Plant Cultivation, Academy of Southern Medicine, Yunnan University of Chinese Medicine, Yunnan, 650500, China.

出版信息

Arch Virol. 2023 Jan 7;168(2):42. doi: 10.1007/s00705-022-05665-7.

Abstract

The complete genome sequence of a putative novel potyvirus, tentatively named "polygonatum mosaic-associated virus 1" (PMaV1), was sequenced from naturally infected Polygonatum cyrtonema Hua in China. PMaV1 has a typical genome organization of potyviruses with a single large open reading frame (nt 119-9448) that encodes a 3109-aa polyprotein that is predicted to be cleaved into 10 mature proteins by virus-encoded proteases. Pairwise comparisons revealed that PMaV1 shares 71.50% complete genome sequence identity with Polygonatum kingianum virus 4 and 80.00% amino acid sequence identity with Polygonatum kingianum virus 3 of the genus Potyvirus. Phylogenetic analysis indicated that PMaV1 clustered with other potyviruses and that it was most closely related to Polygonatum kingianum virus 3 and Polygonatum kingianum virus 4. These results suggest that PMaV1 is a new member of the genus Potyvirus of the family Potyviridae (Nucleotide sequence data reported are available in the GenBank databases under the accession number OP380926).

摘要

从中国自然感染的多花黄精中测序得到了一种假定的新型马铃薯Y病毒的全基因组序列,暂命名为“黄精花叶相关病毒1”(PMaV1)。PMaV1具有典型的马铃薯Y病毒基因组结构,有一个单一的大开放阅读框(核苷酸119 - 9448),编码一个3109个氨基酸的多聚蛋白,预计该多聚蛋白会被病毒编码的蛋白酶切割成10种成熟蛋白。成对比较显示,PMaV1与滇黄精病毒4的全基因组序列同一性为71.50%,与马铃薯Y病毒属的滇黄精病毒3的氨基酸序列同一性为80.00%。系统发育分析表明,PMaV1与其他马铃薯Y病毒聚集在一起,并且与滇黄精病毒3和滇黄精病毒4关系最为密切。这些结果表明,PMaV1是马铃薯Y病毒科马铃薯Y病毒属的一个新成员(报道的核苷酸序列数据可在GenBank数据库中获取,登录号为OP380926)。

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