Lim Seungmo, Lee Su-Heon, Moon Jae Sun
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Arch Virol. 2019 Feb;164(2):621-624. doi: 10.1007/s00705-018-4078-1. Epub 2018 Nov 8.
The complete 9,556-nt genomic sequence of a putative new potyvirus, tentatively named "platycodon mild mottle virus" (PlaMMV), infecting Platycodon grandiflorum was determined (GenBank accession no. MH779625). A single large open reading frame (ORF; nt 121-9360, 3079 aa) encoding a polyprotein that was predicted to be cleaved into 10 mature proteins was detected. By comparison with other potyviruses, nine cleavage sites and conserved domains/motifs were identified. PlaMMV also has a highly conserved GA motif (nt 2857-2863) that could enable a small overlapping ORF (PIPO) embedded within the P3 cistron to be translated by a viral polymerase slippage mechanism. A BLAST analysis showed that the complete PlaMMV nucleotide sequence shared 70% identity (40% query coverage) with carrot thin leaf virus (JX156434) and that the complete amino acid sequence of the PlaMMV polyprotein shared 54% identity (98% query coverage) with Thunberg fritillary mosaic virus (CAI59123). These results suggest that PlaMMV is a new member of the genus Potyvirus in the family Potyviridae.
确定了一种感染桔梗的假定新型马铃薯Y病毒的完整9556个核苷酸的基因组序列，暂命名为“桔梗轻度斑驳病毒”（PlaMMV；GenBank登录号MH779625）。检测到一个单一的大开放阅读框（ORF；核苷酸121 - 9360，3079个氨基酸），其编码一种多聚蛋白，预计该多聚蛋白可被切割成10种成熟蛋白。通过与其他马铃薯Y病毒比较，鉴定出9个切割位点和保守结构域/基序。PlaMMV还具有一个高度保守的GA基序（核苷酸2857 - 2863），该基序可使嵌入P3顺反子内的一个小重叠开放阅读框（PIPO）通过病毒聚合酶滑动机制进行翻译。BLAST分析表明，PlaMMV完整核苷酸序列与胡萝卜细叶病毒（JX156434）的序列一致性为70%（查询覆盖率40%），PlaMMV多聚蛋白的完整氨基酸序列与浙贝母花叶病毒（CAI59123）的序列一致性为54%（查询覆盖率98%）。这些结果表明PlaMMV是马铃薯Y病毒科马铃薯Y病毒属的一个新成员。
