Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Arch Microbiol. 2021 Nov;203(9):5437-5443. doi: 10.1007/s00203-021-02528-8. Epub 2021 Aug 17.
A Gram stain-negative, aerobic, motile by gliding, rod-shaped bacterial strain CAU 1569 was isolated from marine sediment on Shido Island in Incheon. It grew at 20-37 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, 7.0), 2-6% NaCl (w/v) (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CAU 1569 formed a distinct lineage with only the type strain of Snuella. Strain CAU 1569 showed high similarity to S. lapsa KACC 14152 (95.8%), Mariniflexile gromovii KMM KCTC 12570, Aestuariibaculum marinum KCTC 52521 (95.4%), A. suncheonense KACC 16186 (94.6%) and Yeosuana aromativorans KCCM 42019 (94.4%). The genome contained 57 contigs, 3,437 protein-coding gene, 3 rRNAs (5, 16, and 23S), 43 tRNAs, and with a 35.7 mol% G + C content. The DDH value between strain CAU 1569 and S. lapsa KACC 14152 was 39.4 ± 0.6%. The only isoprenoid quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C Ciso G, and C iso 3-OH. Strain CAU 1569 contained diphosphatidylglycerol, aminoglycolipid, unidentified aminolipid, and three unidentified lipids. Based on phylogenetic, genomic, physiologic, and chemotaxonomic characterizations, strain CAU 1569 represents a novel Snuella species, which the name Snuella sedimenti sp. nov. is proposed. The type of strain is CAU 1569 (= KCTC 82409 = MCCC 1K05670).
从仁川市新浦岛的海洋沉积物中分离到一株革兰氏阴性、需氧、能通过滑行运动的杆状细菌 CAU 1569。它在 20-37°C（最佳温度 30°C）、pH 值 6.0-9.0（最佳 pH 值 7.0）、2-6%NaCl（w/v）（最佳 2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CAU 1569与 Snuella 的模式菌株形成了一个独特的分支。菌株 CAU 1569与 Snuella lapsa KACC 14152（95.8%）、Mariniflexile gromovii KMM KCTC 12570、Aestuariibaculum marinum KCTC 52521（95.4%）、A. suncheonense KACC 16186（94.6%）和 Yeosuana aromativorans KCCM 42019（94.4%）的相似度较高。基因组包含 57 个连续片段，3437 个蛋白编码基因，3 个 rRNA（5、16 和 23S），43 个 tRNA，GC 含量为 35.7mol%。菌株 CAU 1569 和 Snuella lapsa KACC 14152 之间的 DDH 值为 39.4±0.6%。唯一的类异戊二烯醌是甲萘醌 6（MK-6）。主要脂肪酸为 iso-C Ciso G 和 C iso 3-OH。菌株 CAU 1569 含有二磷脂酰甘油、氨基糖脂、未鉴定的氨基脂和三种未鉴定的脂类。基于系统发育、基因组、生理和化学分类学特征，菌株 CAU 1569 代表了一个新的 Snuella 种，命名为 Snuella sedimenti sp. nov.。模式菌株为 CAU 1569（=KCTC 82409=MCCC 1K05670）。
