Tran Ngan, Tran Minh, Truong Han, Le Ly
School of Biotechnology, International University-Vietnam National University, Ho Chi Minh 700000, Vietnam.
Foods. 2020 Jul 4;9(7):881. doi: 10.3390/foods9070881.
The present study was performed to spray-dry the high concentration of bioactive compounds from L. extracts that have antidiabetic activity. The total phenolic content (TPC) and total flavonoid content (TFC) of four different extracts (crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract) from the dried powder of L. were determined using a spectrophotometer. After that, the fragment containing a high number of bioactive compounds underwent spray-dried microencapsulation to produce powder which had antidiabetic potential. The total phenolic content values of the crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract were 194.55 ± 0.82, 51.85 ± 3.12, 81.56 ± 1.72 and 214.21 ± 2.53 mg/g extract, expressed as gallic acid equivalents. Crude extract, petroleum ether extract, chloroform extract and ethyl acetate extracts showed total flavonoids 40.56 ± 7.27, 29.49 ± 1.66, 64.99 ± 2.60 and 91.69 ± 1.67 mg/g extract, as rutin equivalents. Ethyl acetate extract was mixed with 20% maltodextrin in a ratio of 1:10 to spray-dry microencapsulation. The results revealed that the moisture content, bulk density, color characteristic, solubility and hygroscopicity of the samples were 4.9567 ± 0.00577%, 0.3715 ± 0.01286 g/mL, 3.7367 ± 0.1424 Hue, 95.83 ± 1.44% and 9.9890 ± 1.4538 g HO/100 g, respectively. The spray powder was inhibited 51.19% α-amylase at 10 mg/mL and reduced 51% in fast blood glucose (FBG) after 4 h treatment. Furthermore, the administration of spray powder for 15 days significantly lowered the fast blood glucose level in streptozotocin-diabetic mice by 23.32%, whereas, acarbose-a standard antidiabetic drug-and distilled water reduced the fast blood glucose level by 30.87% and 16.89%. Our results show that obtained L. powder has potential antidiabetic activity.
本研究旨在对具有抗糖尿病活性的L.提取物中的高浓度生物活性化合物进行喷雾干燥。使用分光光度计测定了L.干粉中四种不同提取物（粗提物、石油醚提取物、氯仿提取物和乙酸乙酯提取物）的总酚含量（TPC）和总黄酮含量（TFC）。之后，对含有大量生物活性化合物的片段进行喷雾干燥微胶囊化，以制备具有抗糖尿病潜力的粉末。粗提物、石油醚提取物、氯仿提取物和乙酸乙酯提取物的总酚含量值分别为194.55±0.82、51.85±3.12、81.56±1.72和214.21±2.53mg/g提取物，以没食子酸当量表示。粗提物、石油醚提取物、氯仿提取物和乙酸乙酯提取物的总黄酮含量分别为40.56±7.27、29.49±1.66、64.99±2.60和91.69±1.67mg/g提取物，以芦丁当量表示。将乙酸乙酯提取物与20%的麦芽糊精按1:10的比例混合进行喷雾干燥微胶囊化。结果表明，样品的水分含量、堆积密度、颜色特征、溶解度和吸湿性分别为4.9567±0.00577%、0.3715±0.01286g/mL、3.7367±0.1424色调、95.83±1.44%和9.9890±1.4538g HO/100g。喷雾粉末在10mg/mL时对α-淀粉酶的抑制率为51.19%，处理4小时后快速血糖（FBG）降低了51%。此外，连续15天给予喷雾粉末可使链脲佐菌素诱导的糖尿病小鼠的快速血糖水平显著降低23.32%，而标准抗糖尿病药物阿卡波糖和蒸馏水使快速血糖水平分别降低了30.87%和16.89%。我们的结果表明，所获得的L.粉末具有潜在的抗糖尿病活性。
