Kimmig B, Lehmann-Grube F
J Gen Virol. 1979 Dec;45(3):703-10. doi: 10.1099/0022-1317-45-3-703.
A search was made in LCM virus-immune mice for virus-specific antibodies. With the help of an L-cell plaque assay, neutralizing antibody was readily detected. There were no essential differences between mouse strains, but marked differences existed between virus strains. Whereas the inoculation of either large or small doses of WE strain virus led to the early production of considerable concentrations of neutralizing antibody, in the case of E-350 strain virus, high doses were required and a much longer time interval had to elapse before the threshold of detection was attained. In addition to neutralizing antibody, LCM virus-infected mice produced sensitizing antibody (detected by the enhancing effect of an anti-mouse Ig antiserum on the ability of a serum to reduce virus infectivity) and complement-fixing antibody. Previous failures to detect neutralizing antibody in LCM virus-immune mice might have been caused by properties of the chosen virus, but in many instances lack of a suitable assay host is a more likely explanation.
在感染淋巴细胞性脉络丛脑膜炎病毒（LCM病毒）的小鼠体内寻找病毒特异性抗体。借助L细胞空斑试验，很容易检测到中和抗体。不同小鼠品系之间没有本质差异，但不同病毒株之间存在显著差异。接种大剂量或小剂量的WE株病毒均可导致早期产生相当浓度的中和抗体，而对于E - 350株病毒，需要高剂量且要经过更长的时间间隔才能达到检测阈值。除中和抗体外，感染LCM病毒的小鼠还产生致敏抗体（通过抗小鼠Ig抗血清对血清降低病毒感染性能力的增强作用来检测）和补体结合抗体。之前在感染LCM病毒的小鼠体内未能检测到中和抗体，可能是所选病毒的特性所致，但在许多情况下，缺乏合适的检测宿主更有可能是原因所在。
