College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Hongshan District, Wuhan, 430070, Hubei Province, People's Republic of China.
Department of Surgery and Pharmacology, Agriculture and Forestry University, Bharatpur, Nepal.
Sci Rep. 2020 Jul 7;10(1):11188. doi: 10.1038/s41598-020-67702-2.
Electroacupuncture (EA) relieves visceral hypersensitivity (VH) with underlying inflammatory bowel diseases. However, the mechanism by which EA treats ileitis-induced VH is not clearly known. To assess the effects of EA on ileitis-induced VH and confirm whether EA attenuates VH through spinal PAR-2 activation and CGRP release, goats received an injection of 2,4,6-trinitro-benzenesulfonic-acid (TNBS) solution into the ileal wall. TNBS-injected goats were allocated into VH, Sham acupuncture (Sham-A) and EA groups, while goats treated with saline instead of TNBS solution were used as the control. Goats in EA group received EA at bilateral Hou-San-Li acupoints for 0.5 h at 7 days and thereafter repeated every 3 days for 6 times. Goats in the Sham-A group were inserted with needles for 0.5 h at the aforementioned acupoints without any hand manipulation and electric stimulation. Visceromotor responses to colorectal distension, an indicator of VH, were recorded by electromyography. The terminal ileum and thoracic spinal cord (T) were sampled for evaluating ileitis at days 7 and 22, and distribution and expression-levels of PAR-2, CGRP and c-Fos on day 22. TNBS-treated-goats exhibited apparent transmural-ileitis on day 7, microscopically low-grade ileitis on day 22 and VH at days 7-22. Goats of Sham-A, VH or EA group showed higher (P < 0.01) VH at days 7-22 than the Control-goats. EA-treated goats exhibited lower (P < 0.01) VH as compared with Sham-A or VH group. Immunoreactive-cells and expression-levels of spinal PAR-2, CGRP and c-Fos in the EA group were greater (P < 0.01) than those in the Control group, but less (P < 0.01) than those in Sham-A and VH groups on day 22. Downregulation of spinal PAR-2 and CGRP levels by EA attenuates the ileitis and resultant VH.
电针对伴有潜在炎症性肠病的内脏高敏性（VH）具有缓解作用。然而，电针治疗回肠炎诱导的 VH 的机制尚不清楚。为了评估电针对回肠炎诱导的 VH 的影响，并确认电针是否通过脊髓 PAR-2 激活和 CGRP 释放来减轻 VH，我们给山羊的回肠壁注射了 2,4,6-三硝基苯磺酸（TNBS）溶液。将注射了 TNBS 的山羊分为 VH、假针刺（Sham-A）和电针（EA）组，而用生理盐水代替 TNBS 溶液处理的山羊则作为对照。EA 组的山羊在第 7 天和之后的每 3 天接受双侧后三里穴位的 EA 治疗 0.5 小时。Sham-A 组的山羊在上述穴位插入针进行 0.5 小时的治疗，但不进行任何手法操作和电刺激。通过肌电图记录内脏运动反应，作为 VH 的指标。在第 7 天和第 22 天分别取末端回肠和胸段脊髓（T）取样，评估回肠炎，并在第 22 天评估 PAR-2、CGRP 和 c-Fos 的分布和表达水平。TNBS 处理的山羊在第 7 天表现出明显的回肠壁炎症，第 22 天表现出低级别回肠炎和第 7-22 天的 VH。Sham-A、VH 或 EA 组的山羊在第 7-22 天的 VH 均显著高于对照组（P < 0.01）。与 Sham-A 或 VH 组相比，EA 治疗的山羊的 VH 显著降低（P < 0.01）。第 22 天，EA 组脊髓 PAR-2、CGRP 和 c-Fos 的免疫反应细胞和表达水平均显著高于对照组（P < 0.01），但显著低于 Sham-A 组和 VH 组（P < 0.01）。脊髓 PAR-2 和 CGRP 水平的下调通过 EA 减轻了回肠炎和由此产生的 VH。
