DAS Kuntal, Khan M Saifulla, Sounder James, Mohan Usha, Prasad S Venkatesh
Krupanidhi College of Pharmacy Department of Pharmacognosy and Phytochemistry, Bangalore, India.
Turk J Pharm Sci. 2020 Jun;17(3):319-328. doi: 10.4274/tjps.galenos.2019.47135. Epub 2020 Jun 22.
To evaluate the presence of phytochemicals in and the antidiabetic activity of aqueous extract of (AEDN) leaf.
Either sex rats were grouped into 5 classes. Alloxan monohydrate and glibenclamide were used as diabetes induction drug and standard drug, respectively. Aqueous extract of the endangered medicinal plant DN was used in two different doses. Diabetes was induced with alloxan monohydrate at 150 mg/kg b.w. The AEDN was standardized with pharmacognostic and phytochemical screening and a chemical test confirmed the presence of phytoconstituents like glycoside, alkaloid, phenols, and flavonoids. Acute toxicity was evaluated for dose selection in an antidiabetic study.
Glibenclamide (5 mg/kg b.w.) and AEDN (200 and 400 mg) were given to all rats with induced diabetes. The reduced blood glucose level may be correlated with the presence of plant secondary metabolites (phenolic compounds), which was identified by thin layer chromatography and confirmed by high performance liquid chromatography studies. The decreased levels of serum total cholesterol, triglyceride, and liver enzyme activity showed the dose dependency of AEDN extract. An oral glucose tolerance test was performed after administration of 200 and 400 mg of AEDN and 5 mg of glibenclamide to different groups, which showed significantly lower oral glucose load during blood sample collection. Animal body weight and dose of AEDN extract had a significant effect on the glucose level in blood (p<0.01).
The first report on the phytochemicals and therapeutic activity of AEDN leaf showed potential antidiabetic activity by increased insulin secretion via enhanced peripheral glucose utilization mechanism.
评估濒危药用植物（AEDN）叶水提取物中的植物化学物质及其抗糖尿病活性。
将雌雄大鼠分为5组。分别使用一水合四氧嘧啶和格列本脲作为糖尿病诱导药物和标准药物。濒危药用植物DN的水提取物以两种不同剂量使用。以150mg/kg体重的一水合四氧嘧啶诱导糖尿病。通过生药学和植物化学筛选对AEDN进行标准化，化学测试证实了糖苷、生物碱、酚类和黄酮类等植物成分的存在。在抗糖尿病研究中评估急性毒性以选择剂量。
给所有诱导糖尿病的大鼠给予格列本脲（5mg/kg体重）和AEDN（200和400mg）。血糖水平降低可能与植物次生代谢产物（酚类化合物）的存在有关，通过薄层色谱法鉴定并经高效液相色谱研究证实。血清总胆固醇、甘油三酯和肝酶活性水平的降低显示了AEDN提取物的剂量依赖性。对不同组给予200和400mg的AEDN以及5mg的格列本脲后进行口服葡萄糖耐量试验，结果显示在采集血样期间口服葡萄糖负荷显著降低。动物体重和AEDN提取物剂量对血糖水平有显著影响（p<0.01）。
关于AEDN叶植物化学物质和治疗活性的首次报告表明，通过增强外周葡萄糖利用机制增加胰岛素分泌，具有潜在的抗糖尿病活性。
