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提取物的气相色谱/质谱分析及其与银纳米颗粒对生长和黄曲霉毒素产生的活性。

GC/MS analysis of extract and its activity with silver nanoparticles against growth and aflatoxins production.

作者信息

Abdelghany T M, Hassan Maryam M, El-Naggar Medhat A

机构信息

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

Biology Department, Faculty of Science, Jazan University, Jazan, Saudi Arabia.

出版信息

Biotechnol Rep (Amst). 2020 Jun 25;27:e00496. doi: 10.1016/j.btre.2020.e00496. eCollection 2020 Sep.

DOI:10.1016/j.btre.2020.e00496
PMID:32637346
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7327896/
Abstract

From ancient to currently, it has been hard to prevent the exposure to mycotoxigenic fungi, due to these fungi occurs naturally in the environment. This paper reports the antifungal activities of the stem extract with silver nanoparticles (AgNPs) against growth and aflatoxins production. Numerous constituents of extract were detected by GC/MS analysis. Methanolic extract at 30, 60 and 90 mg/mL inhibited the growth of , where the inhibition reached to 50.86, 51.60 and 52.58 %. respectively while weak inhibition was observed using the aqueous extract. Growth of was reduced using AgNPs, the highest inhibition 39.31 % was recorded at 100 ppm AgNPs. Synergistic activity was observed by applying 50 ppm of AgNPs with aqueous and methanolic extracts of . A reduction in aflatoxin B and G synthesis was observed using different concentrations of methanolic stems extract of . particularly with AgNPs.

摘要

从古至今,由于产毒真菌在环境中自然存在,一直难以防止接触到它们。本文报道了含银纳米颗粒(AgNPs)的茎提取物对其生长和黄曲霉毒素产生的抗真菌活性。通过气相色谱/质谱分析检测到提取物的多种成分。30、60和90 mg/mL的甲醇提取物抑制了其生长,抑制率分别达到50.86%、51.60%和52.58%,而水提取物的抑制作用较弱。使用AgNPs可降低其生长,在100 ppm AgNPs时记录到最高抑制率39.31%。将50 ppm的AgNPs与该植物的水提取物和甲醇提取物一起使用时观察到协同活性。使用不同浓度的该植物甲醇茎提取物可观察到黄曲霉毒素B和G合成的减少,特别是与AgNPs一起使用时。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/a177216211c1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/c1db95e3c8a7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/2ee9e30ba5e9/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/bbc413e4b832/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/a177216211c1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/c1db95e3c8a7/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/2ee9e30ba5e9/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/bbc413e4b832/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98a7/7327896/a177216211c1/gr4.jpg

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