Cao Xuan, Yang Shan, Cao Chunyang, Zhou Yongjin J
Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, PR China.
Dalian Key Laboratory of Energy Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, PR China.
Synth Syst Biotechnol. 2020 Jul 1;5(3):179-186. doi: 10.1016/j.synbio.2020.06.005. eCollection 2020 Sep.
Current yeast metabolic engineering in isoprenoids production mainly focuses on rewiring of cytosolic metabolic pathway. However, the precursors, cofactors and the enzymes are distributed in various sub-cellular compartments, which may hamper isoprenoid biosynthesis. On the other side, pathway compartmentalization provides several advantages for improving metabolic flux toward target products. We here summarize the recent advances on harnessing sub-organelle for isoprenoids biosynthesis in yeast, and analyze the knowledge about the localization of enzymes, cofactors and metabolites for guiding the rewiring of the sub-organelle metabolism. This review may provide some insights for constructing efficient yeast cell factories for production of isoprenoids and even other natural products.
目前酵母在类异戊二烯生产中的代谢工程主要集中在细胞质代谢途径的重新布线。然而，前体、辅因子和酶分布在不同的亚细胞区室中，这可能会阻碍类异戊二烯的生物合成。另一方面，途径区室化对于提高目标产物的代谢通量具有若干优势。我们在此总结了利用酵母亚细胞器进行类异戊二烯生物合成的最新进展，并分析了有关酶、辅因子和代谢物定位的知识，以指导亚细胞器代谢的重新布线。本综述可能为构建用于生产类异戊二烯乃至其他天然产物的高效酵母细胞工厂提供一些见解。
