Keeler G H, Stankewich J P, Girard A E, Rash J J
Quality Control Division, Pfizer Inc., Groton, Connecticut 06340.
Antimicrob Agents Chemother. 1988 Sep;32(9):1347-9. doi: 10.1128/AAC.32.9.1347.
A quantitative cylinder plate bioassay for sulbactam has been developed which can detect concentrations as low as 0.01 microgram/g in bovine muscle, fat, kidney, and liver tissues. This procedure may also be applicable to human tissues and fluids. In addition to the improved sensitivity, this method differs from all previously described systems because it is based on inhibition by sulbactam of a cell-free beta-lactamase incorporated in the assay agar. The assay is unaffected by the presence of ampicillin even at concentrations 10 times that of the sulbactam concentration. This report describes the analytical technique as well as the accuracy and precision of the method. The assay can be applied to tissue depletion studies.
已开发出一种用于舒巴坦的定量圆筒平板生物测定法，该方法可检测牛肌肉、脂肪、肾脏和肝脏组织中低至0.01微克/克的浓度。该程序也可能适用于人体组织和体液。除了提高灵敏度外，该方法与所有先前描述的系统不同，因为它基于舒巴坦对掺入测定琼脂中的无细胞β-内酰胺酶的抑制作用。即使氨苄青霉素的浓度是舒巴坦浓度的10倍，该测定也不受其存在的影响。本报告描述了分析技术以及该方法的准确性和精密度。该测定法可应用于组织耗竭研究。
