Rajizadeh Mohammad Amin, Najafipour Hamid, Samareh Fekr Mitra, Rostamzadeh Farzaneh, Jafari Elham, Bejeshk Mohammad Abbas, Masoumi-Ardakani Yaser
Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences and Department of Physiology, Afzalipour Medical Faculty, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Pharm Res. 2019 Summer;18(3):1488-1498. doi: 10.22037/ijpr.2019.1100749.
The aim of the present study was to investigate the effect of Myrtenol, the active ingredient of Myrtle on the oxidant and anti-oxidant indices and cytokines in the allergic asthma. Allergic asthma was induced by ovalbumin (OVA) sensitization and inhalation in four groups of rats; Control, Asthma, Asthma + Dexamethasone and Asthma + Myrtenol. Myrtenol (50mg/kg) or Dexamethasone (2.5mg/kg) was administered intraperitoneally for 7 consecutive days after OVA inhalation. At the end, histopathological parameters, and interleukins (Interleukin-10 (IL10), Interferon gamma (IFN-γ) , interleukin-1β (IL-1β), Tumor Necrosis Factor α (TNF-α)), and oxidative stress biomarkers, Malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX) in the lung and serum were measured by hematoxylin and eosin staining and ELISA method, respectively. Myrtenol reduced the pathological changes in the lungs and airway endothelium ( < 0.01), ( < 0.5). The level of IL-1β ( < 0.05) and MDA in the serum and lung tissue ( < 0.01), ( < 0.05), and also the level of TNF-α ( < 0.05) in the lung tissue decreased in the Myrtenol group compared to the asthma group. Myrtenol increased the level of IL-10 ( < 0.05) and the activity of GPX in the lung tissue and serum ( < 0.001). Myrtenol may improve asthma by increasing the ratio of antioxidants to oxidants and reducing the ratio of pro-inflammatory to anti-inflammatory interleukins in the lung. Myrtenol is presented as a potent herbal medicine ingredient for the treatment of asthma.
本研究的目的是探讨桃金娘醇（桃金娘的活性成分）对过敏性哮喘中氧化和抗氧化指标以及细胞因子的影响。通过对四组大鼠进行卵清蛋白（OVA）致敏和吸入诱导过敏性哮喘；对照组、哮喘组、哮喘+地塞米松组和哮喘+桃金娘醇组。在吸入OVA后连续7天腹腔注射桃金娘醇（50mg/kg）或地塞米松（2.5mg/kg）。最后，分别通过苏木精和伊红染色以及ELISA法测量肺和血清中的组织病理学参数、白细胞介素（白细胞介素-10（IL10）、干扰素γ（IFN-γ）、白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α））以及氧化应激生物标志物丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）。桃金娘醇减轻了肺和气道内皮的病理变化（<0.01），（<0.5）。与哮喘组相比，桃金娘醇组血清和肺组织中IL-1β水平（<0.05）和MDA水平（<0.01），（<0.05），以及肺组织中TNF-α水平（<0.05）均降低。桃金娘醇增加了肺组织和血清中IL-10水平（<0.05）和GPX活性（<0.001）。桃金娘醇可能通过增加肺中抗氧化剂与氧化剂的比例以及降低促炎与抗炎白细胞介素的比例来改善哮喘。桃金娘醇是一种用于治疗哮喘的有效草药成分。
