Loncaric Igor, Misic Dusan, Szostak Michael P, Künzel Frank, Schäfer-Somi Sabine, Spergser Joachim
Institute of Microbiology, University of Veterinary Medicine, 1210 Vienna, Austria.
Faculty of Biotechnology and Food Science, Wroclaw University of Environmental and Life Sciences, 51-630 Wrocław, Poland.
Antibiotics (Basel). 2020 Jul 7;9(7):387. doi: 10.3390/antibiotics9070387.
The aim of the present study was to characterize resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains ( ( = 28), ( = 3), , , , and (each = 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. () isolates were additionally characterized by two-locus (C and H) sequence phylotyping and multi-locus sequence typing (MLST) of selected isolates. MLST of carbapenem-resistant isolates was also performed. Among , the most dominant phylogenetic group was B1 (27.8%), followed by C, (16.6%), A and Clade II (5.5% each), B2 and F (2.77% each). The most predominant β-lactam resistance genes were (70%) and (38.8%), (25%). was detected in one carbapenem-resistant ST114. The most common ST among selected was 744 (10.7% isolates). The pandemic clones ST131 and ST648 carrying CTX-M-15 were also detected. Remaining STs belonged to 469, 1287, 1463 and 1642. clonotyping revealed 20 CH types. Based on the presence of certain virulence genes, three isolates were categorized as ExPEC/UPEC. The most prevalent virulence factors were H detected in 61%, D and both in 55%, N in 27.8%, C in 13.8% and in 8.3% isolates.
本研究的目的是对从患有泌尿生殖系统感染的犬猫中分离出的对第三代和第四代头孢菌素、碳青霉烯类和/或氟喹诺酮类耐药的菌株进行特征分析。本研究共纳入36株菌株（大肠埃希菌（n = 28）、肺炎克雷伯菌（n = 3）以及阴沟肠杆菌、产气肠杆菌、摩根氏菌、奇异变形杆菌和普罗威登斯菌（各n = 1）），其中28株来自奥地利，8株来自塞尔维亚。通过多相方法对分离株进行特征分析，包括药敏表型和基因分型以及基于微阵列的检测。部分（n = 22）分离株还通过双位点（C和H）序列系统发育分型以及对选定分离株进行多位点序列分型（MLST）进行特征分析。还对耐碳青霉烯类大肠埃希菌分离株进行了MLST。在大肠埃希菌中，最主要系统发育群是B1（27.8%），其次是C群（16.6%）、A群和进化枝II（各5.5%）、B2群和F群（各2.77%）。最主要的β-内酰胺耐药基因是blaCTX-M（70%）和blaTEM（38.8%）、blaSHV（25%）。在一株耐碳青霉烯类大肠埃希菌ST114中检测到blaNDM-1。选定大肠埃希菌中最常见的ST是744（10.7%的分离株）。还检测到携带CTX-M-15的大流行克隆ST131和ST648。其余ST属于469、1287、1463和1642。大肠埃希菌克隆分型显示有20种CH型。基于某些毒力基因的存在，3株分离株被归类为肠外致病性大肠埃希菌/泌尿道致病性大肠埃希菌（ExPEC/UPEC）。最普遍的毒力因子是HlyA，在61%的分离株中检测到；FimD和PapC均在55%的分离株中检测到；NfaE在27.8%的分离株中检测到；Cnf1在13.8%的分离株中检测到；TraT在8.3%的分离株中检测到。
