Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Asian Health Science Research Institiute, Osaka Prefecture University, Izumisano, Osaka, Japan.
PLoS One. 2021 Feb 5;16(2):e0246482. doi: 10.1371/journal.pone.0246482. eCollection 2021.
The emergence and global spread of extended-spectrum or AmpC β-lactamase (ESBL/AmpC)-producing Enterobacteriaceae in companion animals have led to the hypothesis that companion animals might be reservoirs for cross-species transmission because of their close contact with humans. However, current knowledge in this field is limited; therefore, the role of companion animals in cross-species transmission remains to be elucidated. Herein, we studied ESBL/AmpC-producing Enterobacteriaceae, Escherichia coli in particular, isolated from extraintestinal sites and feces of companion dogs. Whole-genome sequencing analysis revealed that (i) extraintestinal E. coli isolates were most closely related to those isolated from feces from the same dog, (ii) chromosomal sequences in the ST131/C1-M27 clade isolated from companion dogs were highly similar to those in the ST131/C1-M27 clade of human origin, (iii) certain plasmids, such as IncFII/pMLST F1:A2:B20/blaCTX-M-27, IncI1/pMLST16/blaCTX-M-15, or IncI1/blaCMY-2 from dog-derived E. coli isolates, shared high homology with those from several human-derived Enterobacteriaceae, (iv) chromosomal blaCTX-M-14 was identified in the ST38 isolate from a companion dog, and (v) eight out of 14 tested ESBL/AmpC-producing E. coli isolates (i.e., ST131, ST68, ST405, and ST998) belonged to the human extraintestinal pathogenic E. coli (ExPEC) group. All of the bla-coding plasmids that were sequenced genome-wide were capable of horizontal transfer. These results suggest that companion dogs can spread ESBL/AmpC-producing ExPEC via their feces. Furthermore, at least some ESBL/AmpC-producing ExPECs and bla-coding plasmids can be transmitted between humans and companion dogs. Thus, companion dogs can act as an important reservoir for ESBL/AmpC-producing E. coli in the community.
在伴侣动物中出现并在全球传播的扩展谱或 AmpC β-内酰胺酶（ESBL/AmpC）-产生肠杆菌科，导致了这样一种假设，即由于伴侣动物与人类密切接触，它们可能是种间传播的储主。然而，目前在这一领域的知识有限；因此，伴侣动物在种间传播中的作用仍有待阐明。在此，我们研究了从伴侣犬的肠道外部位和粪便中分离出的 ESBL/AmpC 产生的肠杆菌科，特别是大肠杆菌。全基因组测序分析表明：（i）肠道外大肠杆菌分离株与来自同一犬粪便的分离株最密切相关，（ii）从伴侣犬分离出的 ST131/C1-M27 群的染色体序列与人源 ST131/C1-M27 群非常相似，（iii）某些质粒，如来自犬源大肠杆菌分离株的 IncFII/pMLST F1:A2:B20/blaCTX-M-27、IncI1/pMLST16/blaCTX-M-15 或 IncI1/blaCMY-2，与来自几种人源肠杆菌科的质粒高度同源，（iv）在来自伴侣犬的 ST38 分离株中发现了染色体 blaCTX-M-14，（v）在 14 株测试的 ESBL/AmpC 产生大肠杆菌分离株中，有 8 株（即 ST131、ST68、ST405 和 ST998）属于人类肠道外致病性大肠杆菌（ExPEC）组。全基因组测序的所有 bla 编码质粒都能够进行水平转移。这些结果表明，伴侣犬可以通过粪便传播 ESBL/AmpC 产生的 ExPEC。此外，至少一些 ESBL/AmpC 产生的 ExPEC 和 bla 编码质粒可以在人类和伴侣犬之间传播。因此，伴侣犬可以作为社区中 ESBL/AmpC 产生大肠杆菌的重要储主。
