Medical Department 1, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.
Hector-Centre for Nutrition, Exercise and Sports, Medical Department 1, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany.
J Physiol. 2020 Sep;598(18):3871-3889. doi: 10.1113/JP279150. Epub 2020 Jul 28.
Physical activity is known to protect against cancer. The resistance exercise method whole-body electromyostimulation (WB-EMS) has a significant anti-cancer effect. WB-EMS-conditioned serum from advanced prostate cancer patients decreased human prostate carcinoma cell growth and viability in vitro. Multiplex analysis revealed that genes associated with human prostate cancer cell proliferation and apoptosis are sensitive for exercise. Feasible exercise should be part of multimodal anti-cancer therapies, also for physically weakened patients.
Regular physical activity is known to protect against cancer development. In cancer survivors, exercise reduces the risk of cancer recurrence and mortality. However, the link between exercise and decreased cancer risk and improved survival is still not well understood. Serum from exercising healthy individuals inhibits proliferation and activates apoptosis in various cancer cells, suggesting that mechanisms regulating cancer cell growth are affected by exercise. For the first time, we analysed serum from advanced-stage cancer patients with prostate (exercise group n = 8; control group n = 10) or colorectal (exercise n = 6; control n = 6) cancer, after a 12-week whole-body electromyostimulation training (20 min/session, 2×/week; frequency 85 Hz; pulse width 350 µs; 6 s stimulation, 4 s rest), a tolerable, yet effective, resistance exercise for physically weakened patients. We report that serum from these advanced cancer patients inhibits proliferation and enhances apoptosis of human prostate and colon cancer cells in vitro using cell growth and death assays (5-bromo-2'-deoxyuridine incorporation, cell counting, DNA fragmentation). Exercise-mimicking electric pulse stimulation of human primary myotubes showed that electric pulse stimulation-conditioned myotube medium also impairs human cancer cell viability. Gene expression analysis using a multiplex array of cancer-associated genes and subsequent quantitative RT-PCR revealed the presence of exercise-sensitive genes in human prostate cancer cells that potentially participate in the exercise-mediated regulation of malignant cell growth and apoptosis. Our data document the strong efficiency of the anti-oncogenic effects of physical activity and will further support the application of regular therapeutic exercise during cancer disease.
身体活动可预防癌症。阻力运动方法全身肌电刺激（WB-EMS）具有显著的抗癌作用。来自晚期前列腺癌患者的 WB-EMS 调理血清可降低体外人前列腺癌细胞的生长和活力。多重分析显示，与人类前列腺癌细胞增殖和凋亡相关的基因对运动敏感。可行的运动应该是多模式抗癌治疗的一部分，对于身体虚弱的患者也是如此。
有规律的身体活动可预防癌症的发生。在癌症幸存者中，运动可降低癌症复发和死亡的风险。然而，运动与降低癌症风险和提高生存率之间的联系仍不清楚。来自健康个体的运动血清可抑制各种癌细胞的增殖并激活凋亡，表明调节癌细胞生长的机制受到运动的影响。我们首次分析了来自晚期前列腺癌（运动组 n = 8；对照组 n = 10）或结直肠癌（运动 n = 6；对照组 n = 6）患者的血清，这些患者在接受为期 12 周的全身肌电刺激训练（20 分钟/次，2 次/周；频率 85 Hz；脉冲宽度 350 µs；6 s 刺激，4 s 休息）后，这是一种对身体虚弱的患者来说可耐受但有效的阻力运动。我们报告说，这些晚期癌症患者的血清可抑制体外人前列腺和结肠癌细胞的增殖并增强其凋亡，使用细胞生长和死亡测定法（5-溴-2'-脱氧尿苷掺入、细胞计数、DNA 片段化）。对人原代肌管进行模拟运动的电脉冲刺激的实验表明，电脉冲刺激调理的肌管培养基也会损害人癌细胞的活力。使用癌症相关基因的多重分析阵列进行基因表达分析和随后的定量 RT-PCR 显示，人前列腺癌细胞中存在潜在参与运动介导的恶性细胞生长和凋亡调节的运动敏感基因。我们的数据记录了身体活动的强大抗癌效果，并将进一步支持在癌症疾病期间应用常规治疗性运动。
