State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-sen University, Guangzhou, China.
School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
J Appl Microbiol. 2018 Sep;125(3):792-799. doi: 10.1111/jam.13919. Epub 2018 Jun 27.
To reveal the relationship of the bacterial communities in shrimp intestine and surrounding environments.
We examined bacterial communities in the intestine of pacific white shrimp, Litopenaeus vannamei, the surrounding water and sediment by high-throughput sequencing analysis. Sequences were clustered into operational taxonomic units (OTUs) at 97% similarity levels, which ranged from 4956 to 5976 in each sample. All OTUs were affiliated with at least 64 phyla. The 10 most abundant phyla were Proteobacteria, Cyanobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Acidobacteria, Actinobacteria, Planctomycetes, Gemmatimonadetes and Verrucomicrobia. The relationship of bacterial communities in the intestine and the surroundings was also investigated. A total of 1395 OTUs shared in the three habitats, accounting for 80, 65 and 77% in the intestine, the surrounding water and sediment respectively. There were 352, 891, 833 unique OTUs in intestine, surrounding water and sediment. Welch's t-test analysis showed that the abundances of some taxa were significantly different between the shrimp intestine and surroundings. Unweighted pair-group method with arithmetic mean analysis revealed that there was a generally similar bacterial community composition in three environments.
These results showed that the bacterial compositions are mostly the same in shrimp intestine, water and sediment, but with different relative abundances of the bacterial communities.
This study provided valuable findings on the relationship of the bacterial communities in shrimp intestine, the surrounding water and sediment, which can expand our knowledge of the broad trend on bacterial community in shrimp cultural ecosystems.
揭示虾肠道内和周围环境中的细菌群落关系。
通过高通量测序分析，我们检测了太平洋白对虾（Litopenaeus vannamei）肠道内、周围水体和沉积物中的细菌群落。序列在 97%相似水平下聚类为操作分类单元（OTUs），每个样本中的 OTU 数量在 4956 到 5976 之间。所有 OTU 至少与 64 个门有关。丰度最高的 10 个门为变形菌门（Proteobacteria）、蓝藻门（Cyanobacteria）、拟杆菌门（Bacteroidetes）、绿弯菌门（Chloroflexi）、厚壁菌门（Firmicutes）、酸杆菌门（Acidobacteria）、放线菌门（Actinobacteria）、浮霉菌门（Planctomycetes）、芽单胞菌门（Gemmatimonadetes）和疣微菌门（Verrucomicrobia）。还研究了肠道内和周围细菌群落的关系。这三个栖息地共有 1395 个 OTUs 共享，分别占肠道、周围水和沉积物的 80%、65%和 77%。肠道、周围水和沉积物中分别有 352、891、833 个特有 OTUs。Welch 检验分析表明，一些分类群的丰度在虾肠道和周围环境之间存在显著差异。非加权配对组平均法分析显示，三个环境中的细菌群落组成基本相似。
这些结果表明，虾肠道、水和沉积物中的细菌组成大部分相同，但细菌群落的相对丰度不同。
本研究提供了虾肠道内、周围水体和沉积物中细菌群落关系的有价值的发现，可扩展我们对虾养殖生态系统中细菌群落广泛趋势的认识。
