Fouman Faculty of Engineering, College of Engineering, University of Tehran, Iran; Energy, Environment, and Nanostructure Material Laboratory, Caspian Faculty of Engineering, College of Engineering, University of Tehran, Iran.
Energy, Environment, and Nanostructure Material Laboratory, Caspian Faculty of Engineering, College of Engineering, University of Tehran, Iran.
Life Sci. 2020 Sep 15;257:118062. doi: 10.1016/j.lfs.2020.118062. Epub 2020 Jul 9.
In this study, for the first time, the effect of quercetin (Q) on the characteristic properties, antimicrobial activity, and cell viability of polycaprolactone (PCL)/graphene oxide (GO) electrospun scaffold was investigated.
Quercetin loaded graphene oxide nanoparticles have been incorporated into the poly-caprolactone solution, and their mixture has been electrospun to be applied as a nanofibrous scaffold for wound dressing and tissue engineering applications. The properties of scaffolds, like their morphology, tensile strength, hydrophilicity, and in vitro biological performance, are investigated.
The SEM micrographs reveal the uniform bead-free nanofibers with smooth structures have been successfully fabricated via the electrospinning procedure. The overall average of cell viability of NIH/3 T3 fibroblast cells on scaffolds is 95% that means the scaffolds have no toxicity, and FESEM shows cells attach and proliferate on scaffolds. Moreover, among all the fabricated scaffolds, the maximum release of quercetin belongs to PCL/GO/Q 0.5 with about 70% after 15 days, and this scaffold reduces bacterial growth by about 50% after 12 h shows the excellent effect of GO/Q on the antibacterial activity of PCL nanofibers.
The results confirm that more than 1% of GO has some cytotoxicity, which limits its concentration; therefore, a second antibacterial agent is essential to improve the antibacterial activity of PCL/GO scaffold, and quercetin shows that it is an excellent candidate for this purpose.
本研究首次考察了槲皮素（Q）对聚己内酯（PCL）/氧化石墨烯（GO）静电纺丝支架特性、抗菌活性和细胞活力的影响。
将负载有槲皮素的氧化石墨烯纳米粒子掺入聚己内酯溶液中，并将其混合物静电纺丝制成用于伤口敷料和组织工程应用的纳米纤维支架。研究了支架的特性，如形态、拉伸强度、亲水性和体外生物性能。
SEM 显微照片显示，通过静电纺丝工艺成功制备了具有均匀无珠、光滑结构的纳米纤维。NIH/3T3 成纤维细胞在支架上的总体平均细胞活力为 95%，这意味着支架没有毒性，FESEM 显示细胞附着并在支架上增殖。此外，在所有制备的支架中，载有槲皮素的 PCL/GO/Q0.5 在 15 天后的释放量最大，约为 70%，并且该支架在 12 小时后可将细菌生长减少约 50%，表明 GO/Q 对 PCL 纳米纤维的抗菌活性具有优异的效果。
结果证实，超过 1%的 GO 具有一定的细胞毒性，限制了其浓度；因此，需要第二种抗菌剂来提高 PCL/GO 支架的抗菌活性，而槲皮素表明它是这方面的理想候选物。
