Anomasiri W T, Tyrrell D L
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Clin Exp Immunol. 1988 Oct;74(1):59-62.
The production of anti-actin antibodies has been studied in mice. Not one of 10 mice immunized with native actin produced anti-actin antibodies, whereas seven out of 10 mice immunized with actin mixed with paramyxovirus membrane (M) protein produced a high titre of antibodies to actin. The enhancement of anti-actin antibody production by M protein is specific to actin since antibodies to protein purified derivatives (PPD) or gelatin were not enhanced by M protein. The enhanced anti-actin antibodies depended upon the interaction of actin and M protein since injection of M protein and actin in separate sites failed to produce anti-actin antibodies. Five Balb/c nude mice immunized with actin and M protein failed to produce anti-actin antibodies suggesting that cell-mediated immune response is also required for this response.
已在小鼠中研究了抗肌动蛋白抗体的产生。用天然肌动蛋白免疫的10只小鼠中没有一只产生抗肌动蛋白抗体，而用与副粘病毒膜（M）蛋白混合的肌动蛋白免疫的10只小鼠中有7只产生了高滴度的抗肌动蛋白抗体。M蛋白对抗肌动蛋白抗体产生的增强作用对肌动蛋白具有特异性，因为M蛋白不会增强针对蛋白质纯化衍生物（PPD）或明胶的抗体。增强的抗肌动蛋白抗体依赖于肌动蛋白和M蛋白的相互作用，因为在不同部位分别注射M蛋白和肌动蛋白未能产生抗肌动蛋白抗体。用肌动蛋白和M蛋白免疫的5只Balb/c裸鼠未能产生抗肌动蛋白抗体，这表明这种反应也需要细胞介导的免疫反应。
