Pereira Alexandre Godinho, Costa Lidiane Cristina Machado, Soldati Kahena Rodrigues, Guimarães de Abreu Mauro Henrique Nogueira, Costa Fernando Oliveira, Zandim-Barcelos Daniela Leal, Cota Luís Otávio Miranda
Department of Dental Clinics, Oral Pathology, and Oral Surgery,School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
School of Dentistry, Newton Paiva University Center, Minas Gerais, Brazil.
J Int Acad Periodontol. 2020 Jul 1;22(3):90-99.
Human beta-defensins (hBDs) play an important role in the susceptibility to periodontitis. This study aimed to evaluate the levels of hBD-2 and hBD-3 in gingival crevicular fluid (GCF) of individuals with and without periodontitis.
Twenty periodontally healthy individuals (H) and 20 with periodontitis (P) were recruited. GCF samples were collected from healthy sites (Hh; n=20) from H individuals; and from healthy sites (Ph; n=20), sites with gingival inflammation but no attachment loss (Pg; n=20) and sites with inflammation and attachment loss (Pp; n=20) from P individuals. Levels of hBDs (pg/mL) were determined using ELISA. Comparisons between individuals and among sites were performed through hierarchical linear modelling.
GCF levels [median(Q3-Q1)] of hBD-2 were: Hh=4.80 (0.00-30.69); Ph=33.29 (28.04-38.25); Pg=27.56 (23.14-35.50); and Pp=26.20 (23.20-42.54); and of hBD-3 were: Hh=0.00 (0.00-0.00); Ph=978.44 (760.48-1268.12); Pg=938.19 (806.75- 1266.38); and Pp=613.63 (325.50-854.68). Periodontitis at the individual level was associated with higher levels of hBD-2 (p=0.023) and hBD-3 (p greater than 0.001). No influence of site phenotype was observed on hBDs levels.
Individuals with periodontitis presented higher levels of hBD-2 and hBD-3 in the GCF. These levels seemed to be influenced by periodontitis at the individual level but not by periodontal site.
人β-防御素（hBDs）在牙周炎易感性中起重要作用。本研究旨在评估患牙周炎和未患牙周炎个体龈沟液（GCF）中hBD-2和hBD-3的水平。
招募20名牙周健康个体（H组）和20名牙周炎患者（P组）。从H组个体的健康部位（Hh；n = 20）采集GCF样本；从P组个体的健康部位（Ph；n = 20）、有牙龈炎症但无附着丧失的部位（Pg；n = 20）以及有炎症和附着丧失的部位（Pp；n = 20）采集GCF样本。使用酶联免疫吸附测定法（ELISA）测定hBDs（pg/mL）水平。通过分层线性模型对个体间和部位间进行比较。
hBD-2的GCF水平[中位数（四分位距）]为：Hh = 4.80（0.00 - 30.69）；Ph = 33.29（28.04 - 38.25）；Pg = 27.56（23.14 - 35.50）；Pp = 26.20（23.20 - 42.54）；hBD-3的GCF水平为：Hh = 0.00（0.00 - 0.00）；Ph = 978.44（760.48 - 1268.12）；Pg = 938.19（806.75 - 1266.38）；Pp = 613.63（325.50 - 854.68）。个体水平的牙周炎与hBD-2（p = 0.023）和hBD-3（p＞0.001）水平升高相关。未观察到部位表型对hBDs水平有影响。
牙周炎患者的GCF中hBD-2和hBD-3水平较高。这些水平似乎受个体水平的牙周炎影响，而非牙周部位影响。
