Yilmaz Dogukan, Yilmaz Neslihan, Polat Recep, Nissilä Verneri, Aydın Elif Gül, Rautava Jaana, Gürsoy Mervi, Gürsoy Ulvi Kahraman
Department of Periodontology, Faculty of Dentistry, Sakarya University, Sakarya, Turkey.
Department of Pediatric Dentistry, Faculty of Dentistry, Sakarya University, Sakarya, Turkey.
Clin Oral Investig. 2022 Jul;26(7):4897-4904. doi: 10.1007/s00784-022-04457-2. Epub 2022 Mar 21.
Type 1 diabetes mellitus (T1DM), a chronic autoimmune disease characterized by insulin deficiency, is related to periodontal diseases in children and adolescents. Our aim was to profile salivary human beta-defensin (hBD)-2 and hBD-3 concentrations in relation to periodontal and T1DM status in children and adolescent populations.
Unstimulated saliva samples were collected from 66 participants including periodontally healthy T1DM patients (T1DM + C; n = 18), T1DM patients with gingivitis (T1DM + G; n = 20), systemically and periodontally healthy individuals (SH + C: n = 15), and systemically healthy gingivitis patients (SH + G; n = 13). Full mouth plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL) were recorded. Salivary hBD-2 and hBD-3 concentrations were evaluated by sandwich ELISA method. A p value of < 0.05 was considered statistically significant.
Salivary hBD-3 concentrations were lower in T1DM groups in comparison to systemically healthy counterparts (SH + G vs. T1DM + G; p < 0.001 and SH + C vs. T1DM + C; p < 0.001). Salivary hBD-2 levels did not differ between related groups. The difference in hBD-3 concentrations between T1DM and control groups was still significant (p = 0.008) after being adjusted for PI%, BOP%, and age.
In the limits of study, T1DM patients were found to have decreased salivary hBD-3 concentrations, regardless of their gingival inflammatory status.
Altered salivary hBD-3 concentration can partly explain why diabetic children are more prone to periodontal diseases.
1型糖尿病（T1DM）是一种以胰岛素缺乏为特征的慢性自身免疫性疾病，与儿童和青少年的牙周疾病有关。我们的目的是分析儿童和青少年人群中唾液人β-防御素（hBD）-2和hBD-3浓度与牙周和T1DM状况的关系。
收集了66名参与者的非刺激性唾液样本，包括牙周健康的T1DM患者（T1DM + C；n = 18）、患有牙龈炎的T1DM患者（T1DM + G；n = 20）、全身和牙周健康的个体（SH + C：n = 15）以及全身健康的牙龈炎患者（SH + G；n = 13）。记录全口菌斑指数（PI）、探诊出血（BOP）、探诊袋深度（PPD）和临床附着水平（CAL）。通过夹心ELISA法评估唾液hBD-2和hBD-3浓度。p值<0.05被认为具有统计学意义。
与全身健康的对照组相比，T1DM组的唾液hBD-3浓度较低（SH + G与T1DM + G；p < 0.001，SH + C与T1DM + C；p < 0.001）。相关组之间的唾液hBD-2水平没有差异。在调整PI%、BOP%和年龄后，T1DM组和对照组之间hBD-3浓度的差异仍然显著（p = 0.008）。
在本研究范围内，发现T1DM患者的唾液hBD-3浓度降低，无论其牙龈炎症状态如何。
唾液hBD-3浓度的改变可以部分解释为什么糖尿病儿童更容易患牙周疾病。
