Sidharthan Sangamithra, Dharmarajan Gopalakrishnan, Kulloli Anita
Department of Periodontology and Oral Implantology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant Tukaram Nagar, Pimpri, Pune, 411 018, Maharashtra, India.
J Oral Biol Craniofac Res. 2020 Oct-Dec;10(4):498-503. doi: 10.1016/j.jobcr.2020.07.021. Epub 2020 Aug 6.
Interleukin 22(IL-22), a recently identified, Th-22 associated cytokine has a key role in the production of human β defensin-2(hBD-2) and hence an indirect role in innate, nonspecific immunity. Hence this study was conducted as preliminary research to quantify and correlate the levels of IL-22 and hBD-2 in periodontal disease.
Gingival crevicular fluid from subjects with chronic periodontitis (n = 27), gingivitis (n = 25) and healthy controls (n = 27) were obtained for the study. The periodontal status of each subject was assessed by criteria based on plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss. The levels of IL-22 and hBD-2 were measured in GCF samples with an enzyme-linked immunosorbent assay.
Periodontal parameters were described and reported as mean values. The distribution and comparison of clinical parameters and gingival crevicular fluid IL-22, hBD-2 levels in healthy controls, gingivitis and chronic periodontitis groups were assessed using one way ANOVA test. Post Hoc Bonferroni test was used for intergroup comparisons. Association among the IL-22 , hBD-2 and clinical parameters in all three groups were examined using Pearson's correlation test.The IL-22 level was significantly greater in chronic periodontitis group than healthy controls (P < 0.001) and gingivitis group (P < 0.001). The hBD-2 level was significantly higher in the chronic periodontitis group compared to gingivitis (P = 0.003) and healthy controls (P < 0.001). The results also showed a statistically significant correlation (P = 0.002) between IL-22 and hBD-2 concentration in chronic periodontitis group.
These results indicate the role of IL- 22 and hBD-2 in the innate immune response during periodontitis.
白细胞介素22（IL-22）是一种最近发现的与Th-22相关的细胞因子，在人β防御素-2（hBD-2）的产生中起关键作用，因此在先天性非特异性免疫中起间接作用。因此，本研究作为一项初步研究，旨在量化牙周病中IL-22和hBD-2的水平并进行相关性分析。
本研究获取了慢性牙周炎患者（n = 27）、牙龈炎患者（n = 25）和健康对照者（n = 27）的龈沟液。根据菌斑指数、牙龈指数、探诊出血、探诊深度和临床附着丧失等标准评估每位受试者的牙周状况。采用酶联免疫吸附测定法测量龈沟液样本中IL-22和hBD-2的水平。
牙周参数以平均值描述和报告。使用单因素方差分析评估健康对照者、牙龈炎组和慢性牙周炎组临床参数以及龈沟液IL-22、hBD-2水平的分布和比较。采用事后Bonferroni检验进行组间比较。使用Pearson相关检验检查三组中IL-22、hBD-2与临床参数之间的关联。慢性牙周炎组的IL-22水平显著高于健康对照者（P < 0.001）和牙龈炎组（P < 0.001）。与牙龈炎组（P = 0.003）和健康对照者（P < 0.001）相比，慢性牙周炎组的hBD-2水平显著更高。结果还显示慢性牙周炎组中IL-22与hBD-2浓度之间存在统计学显著相关性（P = 0.002）。
这些结果表明IL-22和hBD-2在牙周炎期间的先天性免疫反应中起作用。
