Division of Biomedical Food Research, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
Department of Applied Veterinary Science, the United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
J Vet Med Sci. 2020 Oct 7;82(9):1400-1403. doi: 10.1292/jvms.20-0184. Epub 2020 Jul 10.
Here, we investigated the presence of ST398 livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in nasal swabs of 420 slaughtered pigs from 84 farms at three abattoirs in Tohoku, Japan. MRSA were isolated from 13 (3.1%) samples from 9 (10.7%) farms at two abattoirs. All isolates were classified as ST398 and were resistant to ampicillin and tetracycline. Ten and three isolates were classified as Staphylococcal Cassette Chromosome mec (SCCmec) types V and IVa, respectively. All type V isolates possessed czrC. The minimum inhibitory concentrations (MICs) of zinc chloride against types IVa and V were 1 and 4 mM, respectively. This study shows the presence of ST398 MRSA in pigs in this region. Antimicrobials and zinc compounds in feed and drugs might select SCCmec type V ST398 MRSA.
本研究调查了日本东北地区三个屠宰场的 84 个农场的 420 头屠宰猪的鼻腔拭子中是否存在 ST398 型与牲畜相关的耐甲氧西林金黄色葡萄球菌（LA-MRSA）。从两个屠宰场的 9 个（10.7%）农场的 13 个（3.1%）样本中分离出了 MRSA。所有分离株均被分类为 ST398，且对氨苄西林和四环素具有耐药性。10 个和 3 个分离株分别被分类为 SCCmec 类型 V 和 IVa。所有类型 V 分离株均携带 czrC。锌盐对 IVa 和 V 型的最小抑菌浓度（MIC）分别为 1 和 4mM。本研究表明该地区猪中存在 ST398 型 MRSA。饲料和药物中的抗生素和锌化合物可能会选择 SCCmec 类型 V ST398 MRSA。
