Anjos Mayara Nunes Vitor, Araújo-Neto Luiz Nascimento de, Silva Buonafina Maria Daniela, Pereira Neves Rejane, Souza Edson Rubhens de, Bezerra Isabelle Cristinne Ferraz, Ferreira Magda Rhayanny Assunção, Soares Luiz Alberto Lira, Coutinho Henrique Douglas Melo, Martins Natália, da Silva Márcia Vanusa, Correia Maria Tereza Dos Santos
Laboratory of Natural Products, Department of Biochemistry, Federal University of Pernambuco, Recife 50670-901, Brazil.
Laboratory of Medical Mycology, Department of Mycology, Federal University of Pernambuco, Recife 50670-901, Brazil.
Antibiotics (Basel). 2020 Jul 9;9(7):394. doi: 10.3390/antibiotics9070394.
Opportunistic fungal infections are increasingly common, with being the most common etiological agent; however, in recent years, episodes of candidiasis caused by non- species have emerged. Plants belonging to the Lauraceae family have shown remarkable antifungal effects. This study assessed the anti- activity of extracts and fractions, time of death and the synergistic effects with conventional antifungals. The possible mechanism of action was also addressed.
Minimal inhibitory concentrations (MIC) were determined by broth microdilution technique, and the mechanism of action was assessed by ergosterol, sorbitol, cell viability, reactive oxygen species (ROS) generation and phosphatidylserine externalization tests.
All the tested extracts evidenced antifungal activity, but the methanol extract was revealed to be the most effective (MIC = 3.12 μg/mL) on . The combination of methanol extract with ketoconazole and fluconazole revealed a synergistic effect for and , respectively. Fractions 1 and 5 obtained from the methanol extract had fungicidal activity, mainly against . Methanol extract did not reveal effects by ergosterol and sorbitol assays; however, it led to an increase in intracellular ROS levels, decreased cell viability, and consequently, cell death.
methanol extract may be viewed as a rich source of biomolecules with antifungal activity against spp.
机会性真菌感染日益常见，其中[具体真菌名称]是最常见的病原体；然而，近年来，由非[具体真菌名称]菌种引起的念珠菌病病例已出现。樟科植物已显示出显著的抗真菌作用。本研究评估了[植物名称]提取物和馏分的抗[真菌名称]活性、死亡时间以及与传统抗真菌药物的协同作用。还探讨了可能的作用机制。
采用肉汤微量稀释技术测定最低抑菌浓度（MIC），并通过麦角固醇、山梨醇、细胞活力、活性氧（ROS）生成和磷脂酰丝氨酸外化试验评估作用机制。
所有测试提取物均显示出抗真菌活性，但甲醇提取物对[真菌名称]最有效（MIC = 3.12μg/mL）。甲醇提取物与酮康唑和氟康唑联合使用分别对[两种真菌名称]显示出协同作用。从甲醇提取物中获得的馏分1和馏分5具有杀菌活性，主要针对[真菌名称]。甲醇提取物在麦角固醇和山梨醇试验中未显示出作用；然而，它导致细胞内ROS水平升高，细胞活力降低，进而导致细胞死亡。
甲醇提取物可被视为富含具有抗[真菌名称]菌种抗真菌活性生物分子的来源。
