类风湿性粘连滑膜细胞产生的B细胞分化因子活性可被抗B细胞刺激因子-2/白细胞介素6抗体中和。

Rheumatoid adherent synovial cells produce B cell differentiation factor activity neutralizable by antibody to B cell stimulatory factor-2/interleukin 6.

作者信息

Harigai M, Kitani A, Hara M, Hirose T, Norioka K, Suzuki K, Suzuki M, Kawagoe M, Shinmei M, Nakamura H

机构信息

1st Department of Medicine, National Defense Medical College, Saitama, Japan.

出版信息

J Rheumatol. 1988 Nov;15(11):1616-22.

PMID:3266248

Abstract

B cell differentiation factor (BCDF) activity assayed on SKW6-CL4 cells was found in adherent synovial cell (ASC) conditioned medium. ASC of patients with RA (n = 8) produced significantly larger amounts of BCDF than those of patients with osteoarthritis (n = 5) (219.0 +/- 212.1 vs 25.5 +/- 12.4 units/microgram.DNA, p less than 0.01). ASC clones, established by the limiting dilution technique, also produced this factor. Experiments with several neutralizing antibodies revealed that this BCDF is inhibited by anti-B cell stimulatory factor-2/interleukin 6 antibody (anti-BSF-2/IL-6 ab) up to 90%, but not by antiinterleukin 1, antiinterleukin 2 or antiinterferon-r antibodies. Our data suggest that ASC could participate in the B cell differentiation process in joint space by producing a molecule which has a similar active site to BSF-2/IL-6.

摘要

在SKW6 - CL4细胞上检测到的B细胞分化因子（BCDF）活性存在于贴壁滑膜细胞（ASC）条件培养基中。类风湿性关节炎患者（n = 8）的ASC产生的BCDF量显著高于骨关节炎患者（n = 5）（219.0±212.1对25.5±12.4单位/微克.DNA，p<0.01）。通过有限稀释技术建立的ASC克隆也产生这种因子。用几种中和抗体进行的实验表明，这种BCDF被抗B细胞刺激因子-2/白细胞介素6抗体（抗BSF - 2/IL - 6抗体）抑制高达90%，但不受抗白细胞介素1、抗白细胞介素2或抗干扰素-γ抗体的抑制。我们的数据表明，ASC可能通过产生一种与BSF - 2/IL - 6具有相似活性位点的分子参与关节腔内的B细胞分化过程。