Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China; Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, China.
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China; Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai, China; Graduate University of Chinese Academy of Sciences, Beijing, China.
Food Chem. 2020 Dec 15;333:127478. doi: 10.1016/j.foodchem.2020.127478. Epub 2020 Jul 5.
Moringa oleifera Lam. (M. oleifera) leaves have long been consumed as both nutritive vegetable and popular folk medicine for hyperglycemia and hyperlipidemia in Kenya communities. In the current study, in vitro inhibition by M. oleifera leaf extract (MOLE, 90% (v/v) ethanol) of α-glucosidase and pancreatic lipase was demonstrated, followed by determination of the effects of MOLE on both glucose consumption and lipid levels (TC, TG, HDL-C and LDL-C) in 3T3-L1 cells. Potential ligands in MOLE were fast screened using affinity ultrafiltration LC-MS, and 14 and 10 components displayed certain binding affinity to α-glucosidase and pancreatic lipase, respectively. Docking studies revealed the binding energies and hydrogen bonds between potential ligands and enzymes. This study suggests that M. oleifera leaves may be a promising natural source for the prevention and treatment of hyperglycemia and hyperlipidemia as well as a functional food or other product for health care in the near future.
辣木（Moringa oleifera Lam.）叶在肯尼亚社区中一直被作为营养蔬菜和流行的民间药物用于治疗高血糖和高血脂。在本研究中，证明了辣木叶提取物（MOLE，90%（v/v）乙醇）对α-葡萄糖苷酶和胰脂肪酶的体外抑制作用，随后测定了 MOLE 对 3T3-L1 细胞中葡萄糖消耗和脂质水平（TC、TG、HDL-C 和 LDL-C）的影响。使用亲和超滤 LC-MS 快速筛选 MOLE 中的潜在配体，14 种和 10 种成分分别显示出对 α-葡萄糖苷酶和胰脂肪酶的一定结合亲和力。对接研究揭示了潜在配体与酶之间的结合能和氢键。本研究表明，辣木叶可能是预防和治疗高血糖和高血脂的有前途的天然来源，并且在不久的将来可能成为功能性食品或其他用于保健的产品。
