Ma Shuang, Sun Hao, Yang Weichao, Gao Mingfu, Xu Hui
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Microorganisms. 2020 Jul 11;8(7):1027. doi: 10.3390/microorganisms8071027.
The insulin receptor (InR) pertains to the insulin receptor family, which plays a key role in the insulin/insulin-like growth factor (IGF)-like signaling (IIS) pathway. Insulin signaling defects may result in the development of metabolic diseases, such as type 2 diabetes, and the InR mutant has been suggested to bear insulin signaling deficiency. Numerous studies have reported that probiotics are beneficial for the treatment of diabetes; however, the effect of probiotics on patients with deficiency has seldom been reported. Therefore, we chose the /TM2 to investigate. The results indicated that probiotics significantly reduce the mean and median lifespan of /TM2 (by 15.56% and 23.82%, respectively), but promote that of wild-type files (by 9.31% and 16.67%, respectively). Significant differences were obtained in the expression of lifespan- and metabolism-related genes, such as , , and , between the standard diet groups and the probiotics groups. Furthermore, analysis of 16S rDNA via high throughput sequencing revealed that the gut bacterial diversity of fed with a probiotic combination also differs from that of fed with a standard diet. In summary, these findings indicate that a probiotic combination indeed affects /TM2 Drosophila, but not all of its impacts are positive.
胰岛素受体（InR）属于胰岛素受体家族，在胰岛素/胰岛素样生长因子（IGF）样信号传导（IIS）途径中起关键作用。胰岛素信号缺陷可能导致代谢性疾病的发生，如2型糖尿病，并且已表明InR突变体存在胰岛素信号缺陷。大量研究报告称益生菌对糖尿病治疗有益；然而，益生菌对胰岛素信号缺陷患者的影响鲜有报道。因此，我们选择了InR突变体果蝇（InR/TM2）进行研究。结果表明，益生菌显著缩短了InR/TM2果蝇的平均寿命和中位寿命（分别缩短了15.56%和23.82%），但延长了野生型果蝇的寿命（分别延长了9.31%和16.67%）。在标准饮食组和益生菌组之间，与寿命和代谢相关的基因（如daf-16、foxo和insulin-like peptide 2）的表达存在显著差异。此外，通过高通量测序对16S rDNA进行分析发现，喂食益生菌组合的果蝇肠道细菌多样性也与喂食标准饮食的果蝇不同。总之，这些发现表明益生菌组合确实会影响InR/TM2果蝇，但并非所有影响都是积极的。
