The Foodborne Strain MBC2 Triggers -Dependent Pro-Longevity Effects in .

Lactic acid bacteria (LAB) are involved in several food fermentations and many of them provide strain-specific health benefits. Herein, the probiotic potential of the foodborne strain MBC2 was investigated through in vitro and in vivo approaches. was used as an in vivo model to analyze pro-longevity and anti-aging effects. MBC2 showed a high gut colonization capability compared to OP50 (OP50) or L. rhamnosus GG (LGG). Moreover, analysis of pumping rate, lipofuscin accumulation, and body bending showed anti-aging effects in MBC2-fed worms. Studies on PEPT-1 mutants demonstrated that gene was involved in the anti-aging processes mediated by this bacterial strain through DAF-16, whereas the oxidative stress protection was PEPT-1 independent. Moreover, analysis of acid tolerance, bile tolerance, and antibiotic susceptibility were evaluated. MBC2 exerted beneficial effects on nematode lifespan, influencing energy metabolism and oxidative stress resistance, resulted in being tolerant to acidic pH and able to adhere to Caco-2 cells. Overall, these findings provide new insight for application of this strain in the food industry as a newly isolated functional starter. Furthermore, these results will also shed light on molecular players involved in host-microbe interactions.