Schifano Emily, Zinno Paola, Guantario Barbara, Roselli Marianna, Marcoccia Sante, Devirgiliis Chiara, Uccelletti Daniela
Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Research Centre for Food and Nutrition, CREA (Council for Agricultural Research and Economics), 00178 Rome, Italy.
Microorganisms. 2019 Feb 7;7(2):45. doi: 10.3390/microorganisms7020045.
Lactic acid bacteria (LAB) are involved in several food fermentations and many of them provide strain-specific health benefits. Herein, the probiotic potential of the foodborne strain MBC2 was investigated through in vitro and in vivo approaches. was used as an in vivo model to analyze pro-longevity and anti-aging effects. MBC2 showed a high gut colonization capability compared to OP50 (OP50) or L. rhamnosus GG (LGG). Moreover, analysis of pumping rate, lipofuscin accumulation, and body bending showed anti-aging effects in MBC2-fed worms. Studies on PEPT-1 mutants demonstrated that gene was involved in the anti-aging processes mediated by this bacterial strain through DAF-16, whereas the oxidative stress protection was PEPT-1 independent. Moreover, analysis of acid tolerance, bile tolerance, and antibiotic susceptibility were evaluated. MBC2 exerted beneficial effects on nematode lifespan, influencing energy metabolism and oxidative stress resistance, resulted in being tolerant to acidic pH and able to adhere to Caco-2 cells. Overall, these findings provide new insight for application of this strain in the food industry as a newly isolated functional starter. Furthermore, these results will also shed light on molecular players involved in host-microbe interactions.
乳酸菌（LAB）参与多种食品发酵，其中许多具有菌株特异性的健康益处。在此，通过体外和体内方法研究了食源菌株MBC2的益生菌潜力。 被用作体内模型来分析延长寿命和抗衰老作用。与OP50（OP50）或鼠李糖乳杆菌GG（LGG）相比，MBC2表现出较高的肠道定植能力。此外，对泵送速率、脂褐素积累和身体弯曲的分析表明，喂食MBC2的线虫具有抗衰老作用。对PEPT-1突变体的研究表明， 基因通过DAF-16参与了该菌株介导的抗衰老过程，而氧化应激保护与PEPT-1无关。此外，还评估了耐酸性、耐胆汁性和抗生素敏感性。MBC2对线虫寿命产生有益影响，影响能量代谢和抗氧化应激能力，能够耐受酸性pH并能粘附于Caco-2细胞。总体而言，这些发现为该菌株作为新分离的功能性发酵剂在食品工业中的应用提供了新的见解。此外，这些结果也将揭示宿主-微生物相互作用中涉及的分子机制。
