Center for Translational Medicine and Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
University of Hawaii Cancer Center, Honolulu, 96813, USA.
Sci Rep. 2020 Jul 14;10(1):11601. doi: 10.1038/s41598-020-68393-5.
Bile acids (BAs) play essential roles in facilitating lipid digestion and absorption in the intestine. Gastric BAs were attributed to abnormal refluxing from duodenal compartments and correlated with the occurrence of gastric inflammation and carcinogenesis. However, the differences in gastric BAs between physiologically compromised and healthy individuals have not been fully investigated. In this study, gastric juice was collected from patients clinically diagnosed as gastritis with/without bile reflux and healthy subjects for BA profiles measurements. As a result, we found that the conjugated BAs became prominent components in bile reflux juice, whereas almost equal amounts of conjugated and unconjugated BAs existed in non-bile reflux and healthy juice. To investigate whether gastric BA changes were regulated by hepatic BA synthesis, C57BL/6J mice were intervened with GW4064/resin to decrease/increase hepatic BA synthesis. The results revealed that changes of gastric BAs were coordinated with hepatic BA changes. Additionally, gastric BAs were detected in several healthy mammals, in which there were no obvious differences between the conjugated and unconjugated BAs. Pigs were an exception. Thus, increased levels of conjugated BAs are associated with human bile reflux gastritis. Gastric conjugated BAs could become a panel of biomarkers to facilitate diagnosis of pathological bile reflux.
胆汁酸（BAs）在促进肠道内脂质消化和吸收方面发挥着重要作用。胃胆汁酸归因于十二指肠隔室的异常反流，并与胃炎症和癌变的发生相关。然而，生理功能受损者和健康个体之间胃胆汁酸的差异尚未得到充分研究。在这项研究中，从临床上诊断为胃炎伴/不伴胆汁反流和健康受试者的患者中收集胃液，用于 BA 谱测量。结果发现，结合胆汁酸成为胆汁反流液中的主要成分，而在非胆汁反流和健康胃液中，结合和非结合胆汁酸的含量几乎相等。为了研究胃 BA 的变化是否受肝 BA 合成的调节，用 GW4064/树脂干预 C57BL/6J 小鼠以减少/增加肝 BA 合成。结果表明，胃 BA 的变化与肝 BA 的变化相协调。此外，在几种健康哺乳动物中检测到了胃胆汁酸，其中结合和非结合胆汁酸之间没有明显差异。猪是个例外。因此，结合胆汁酸水平升高与人类胆汁反流性胃炎有关。胃结合胆汁酸可能成为一个有助于诊断病理性胆汁反流的生物标志物组合。
