扩展膜蛋白 NMR 工具包。
Expanding the membrane-protein NMR toolkit.
机构信息
Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA, USA.
出版信息
Nat Chem Biol. 2020 Sep;16(9):937-938. doi: 10.1038/s41589-020-0597-7.
Abstract
An NMR method to monitor conformational states of challenging large protein targets is described. The method, which can be used to evaluate distances between two labels and to measure conformational exchange rates, revealed an unanticipated outward-facing state in a glutamate transporter.
摘要
本文描述了一种用于监测具有挑战性的大型蛋白质靶标构象状态的 NMR 方法。该方法可用于评估两个标记之间的距离，并测量构象交换速率，它揭示了一种谷氨酸转运蛋白的意外外向构象状态。
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