Course of Biological Science and Technology, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
Food Funct. 2020 Jul 1;11(7):5976-5991. doi: 10.1039/d0fo00795a. Epub 2020 Jul 15.
Non-alcoholic fatty liver disease (NAFLD) is a disease that is prevalent worldwide, and its prevention by dietary administration has recently been considered as an important strategy. In this study, we administered mice with vine tea polyphenol (VTP) extracted from Ampelopsis grossedentata, a Chinese herb, to investigate the preventive effect on western diet (WD)-induced NAFLD. Male C57BL/6N mice were fed either a normal diet (ND) or WD with or without VTP for 12 weeks. The results revealed that VTP supplementation decreased the serum levels of cholesterol and triglycerides, and reduced the accumulation of hepatic lipid droplets caused by WD. Molecular data revealed that VTP enhanced fatty acid oxidation by reactivating the WD-suppressed phosphorylation of AMP-activated protein kinaseα (AMPKα) and the expressions of peroxisome proliferator-activated receptor alpha (PPARα), carnitine palmitoyl transferase IA (CPT1A) and cytochrome P450, family 4, subfamily a1 (CYP4A1). VTP inhibited hepatic lipogenesis by reducing the WD-enhanced level of mature sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS). Moreover, VTP activated nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-mediated expressions of hemeoxygenase-1 (HO-1) and quinone oxidoreductase (NQO1), and reduced hepatic TBARS levels to prevent hepatic oxidative stress. On the other hand, VTP also increased intestinal zonula occludens-1 (ZO-1) expression and the relative abundance of gut Akkermansia, and reduced the ratio of Firmicutes/Bacteroidetes. Thus, VTP might prevent WD-induced NAFLD by balancing fatty acid oxidation and lipogenesis, hepatic oxidative stress, and gut microbiome, at least. These results suggest that vine tea, containing a high content of the bioactive compound dihydromyricetin, is a potential food resource for preventing NAFLD.
非酒精性脂肪性肝病（NAFLD）是一种在全球范围内普遍存在的疾病，最近人们认为通过饮食管理来预防这种疾病是一种重要的策略。在这项研究中，我们给小鼠喂食了从中国草药蛇葡萄中提取的藤茶多酚（VTP），以研究其对西方饮食（WD）诱导的非酒精性脂肪性肝病的预防作用。雄性 C57BL/6N 小鼠分别喂食正常饮食（ND）或 WD 饮食，同时或不补充 VTP 喂养 12 周。结果表明，VTP 补充可降低血清胆固醇和甘油三酯水平，并减少 WD 引起的肝脂质滴积累。分子数据显示，VTP 通过重新激活 WD 抑制的 AMP 激活蛋白激酶α（AMPKα）磷酸化和过氧化物酶体增殖物激活受体α（PPARα）、肉碱棕榈酰转移酶 IA（CPT1A）和细胞色素 P450 家族 4 亚家族 A1（CYP4A1）的表达，增强脂肪酸氧化。VTP 通过降低 WD 增强的成熟固醇调节元件结合蛋白 1（SREBP1）和脂肪酸合酶（FAS）水平来抑制肝脂肪生成。此外，VTP 激活核因子（红细胞衍生 2）样 2（Nrf2）介导的血红素加氧酶-1（HO-1）和醌氧化还原酶（NQO1）的表达，并降低肝 TBARS 水平以防止肝氧化应激。另一方面，VTP 还增加了肠道紧密连接蛋白-1（ZO-1）的表达和肠道阿克曼氏菌的相对丰度，并降低了厚壁菌门/拟杆菌门的比例。因此，VTP 至少通过平衡脂肪酸氧化和脂肪生成、肝氧化应激和肠道微生物组来预防 WD 诱导的非酒精性脂肪性肝病。这些结果表明，含有高含量生物活性化合物二氢杨梅素的藤茶可能是预防非酒精性脂肪性肝病的潜在食物资源。
