Shimozawa K, Matsumoto M, Okada K, Murata M, Tsuchiya Y, Kitagawa T
Department of Pediatrics, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Horm Res. 1988;30(6):246-51. doi: 10.1159/000181071.
Blood spot 17 alpha-hydroxyprogesterone (17-OHP) concentrations were measured in 515 infants aged from the 4th to the 81st day of life, using a fluorescence enzyme immunoassay method, and the values obtained were analyzed with respect to birth weight, gestational age at birth and sampling age, to obtain accurate reference ranges and to decide appropriate cut-off limits in a neonatal mass screening for steroid 21-hydroxylase deficiency. The results obtained indicate that the blood spot 17-OHP values in neonates should be interpreted using several different reference ranges obtained on the basis of the equivalent age of gestation at blood sampling. In the mass screening, therefore, the cut-off limits for recall are decided by these reference ranges as follows: (1) for blood resampling, 120, 30 and 25 nmol/l for the equivalent sampling ages of 31 weeks or less, 32-41 weeks and 42 weeks or more, respectively, and (2) for rapid confirmation of the disease by means of physical and laboratory examinations, 210, 60 and 60 nmol/l, respectively.
采用荧光酶免疫分析法测定了515例出生后4天至81天婴儿血斑中17α-羟孕酮（17-OHP）的浓度，并对所获数值按出生体重、出生孕周和采血年龄进行分析，以获取准确的参考范围，并确定新生儿群体筛查类固醇21-羟化酶缺乏症时的合适截断值。所得结果表明，新生儿血斑17-OHP值应根据采血时的等效孕周采用几个不同的参考范围来解释。因此，在群体筛查中，召回的截断值由这些参考范围确定如下：（1）对于再次采血，等效采样年龄31周及以下、32 - 41周和42周及以上时分别为120、30和25 nmol/l；（2）对于通过体格检查和实验室检查快速确诊疾病，分别为210、60和60 nmol/l。
