Seo Seungbeom, Kim Joon, Lee Jun-Woo, Nam Onyou, Chang Kwang Suk, Jin EonSeon
Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, 04763 Republic of Korea.
Biotechnol Biofuels. 2020 Jul 10;13:120. doi: 10.1186/s13068-020-01760-6. eCollection 2020.
The development of microalgal strains for enhanced biomass and biofuel production has received increased attention. Moreover, strain development via metabolic engineering for commercial production is being considered as the most efficient strategy. Pyruvate is an essential metabolite in the cells and plays an essential role in amino acid biosynthesis and de novo fatty acid biosynthesis in plastids. Although pyruvate can be a valuable target for metabolic engineering, its transporters have rarely been studied in microalgae. In this study, we aimed to identify the plastidial pyruvate transporter of and utilize it for strain development.
We identified putative pyruvate transporter localized in the plastid membrane of . Transformants overexpressing the pyruvate transporter were generated to increase the influx of pyruvate into plastids. Overexpression of a plastidial pyruvate transporter in resulted in enhanced biomass (13.6% to 21.9%), lipid contents (11% to 30%), and growth (3.3% to 8.0%) compared to those of wild type during one-stage cultivation.
To regulate the pyruvate influx and its metabolism in plastids, we generated transformants overexpressing the putative plastidial pyruvate transporter in . They showed that its overexpression for compartmentalizing pyruvate in plastids could be an attractive strategy for the effective production of biomass and lipids with better growth, via enhanced pyruvate metabolism in plastids.
开发用于提高生物量和生物燃料产量的微藻菌株已受到越来越多的关注。此外，通过代谢工程进行菌株开发以实现商业生产被认为是最有效的策略。丙酮酸是细胞中的一种必需代谢物，在氨基酸生物合成以及质体中的从头脂肪酸生物合成中起着至关重要的作用。尽管丙酮酸可能是代谢工程的一个有价值的靶点，但在微藻中对其转运蛋白的研究却很少。在本研究中，我们旨在鉴定[具体微藻名称未给出]的质体丙酮酸转运蛋白并将其用于菌株开发。
我们鉴定出了定位于[具体微藻名称未给出]质体膜上的假定丙酮酸转运蛋白。通过过表达丙酮酸转运蛋白产生了转化体，以增加丙酮酸进入质体的通量。与野生型相比，在一阶段培养过程中，[具体微藻名称未给出]中质体丙酮酸转运蛋白的过表达导致生物量提高了13.6%至21.9%，脂质含量提高了11%至30%，生长速率提高了3.3%至8.0%。
为了调节丙酮酸进入质体及其在质体中的代谢，我们在[具体微藻名称未给出]中产生了过表达假定质体丙酮酸转运蛋白的转化体。结果表明，通过增强质体中的丙酮酸代谢，过表达该转运蛋白以将丙酮酸分隔在质体中可能是一种有效的策略，可用于有效生产生物量和脂质并实现更好的生长。
