含有AT碱基对的Z-DNA六聚体d（CACGTG）的结构。
Structure of d(CACGTG), a Z-DNA hexamer containing AT base pairs.
作者信息
Coll M, Fita I, Lloveras J, Subirana J A, Bardella F, Huynh-Dinh T, Igolen J
机构信息
Unidad de Química Macromolecular, Escuela de Ingenieros Industriales, Barcelona, Spain.
出版信息
Nucleic Acids Res. 1988 Sep 12;16(17):8695-705. doi: 10.1093/nar/16.17.8695.
Abstract
The left-handed Z-DNA conformation has been observed in crystals made from the self-complementary DNA hexamer d(CACGTG). This is the first time that a non disordered Z form is found in the crystal structure of an alternating sequence containing AT base pairs without methylated or brominated cytosines. The structure has been determined and refined to an agreement factor R = 22.9% using 746 reflections in the resolution in the resolution shell 7 to 2.5 A. The overall shape of the molecule is very similar to the Z-structure of the related hexamer d(CG)3 confirming the rigidity of the Z form. No solvent molecules were detected in the minor groove of the helix near the A bases. The disruption of the spine of hydration in the AT step appears to be a general fact in the Z form in contrast with the B form. The biological relevance of the structure in relation to the CA genome repeats is discussed.
摘要
左手螺旋的Z-DNA构象已在由自互补DNA六聚体d（CACGTG）形成的晶体中被观察到。这是首次在不含甲基化或溴化胞嘧啶的含AT碱基对的交替序列晶体结构中发现非无序的Z型。该结构已通过分辨率为7至2.5埃的壳层中的746个反射确定并精修至一致性因子R = 22.9%。分子的整体形状与相关六聚体d（CG）3的Z结构非常相似，证实了Z型的刚性。在靠近A碱基的螺旋小沟中未检测到溶剂分子。与B型相比，AT步中氢键主链的破坏似乎是Z型的一个普遍现象。文中讨论了该结构与CA基因组重复序列的生物学相关性。
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