SMC 蛋白复合物介导的 DNA 环伸出的结构见解。
Structural insights into DNA loop extrusion by SMC protein complexes.
机构信息
European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg, Germany; Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Germany.
European Molecular Biology Laboratory (EMBL), Meyerhofstr. 1, 69117 Heidelberg, Germany; Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
出版信息
Curr Opin Struct Biol. 2020 Dec;65:102-109. doi: 10.1016/j.sbi.2020.06.009. Epub 2020 Jul 13.
Abstract
Structural Maintenance of Chromosomes (SMC) protein complexes play key roles in the three-dimensional organization of genomes in all kingdoms of life. Recent insights from chromosome contact mapping experiments and single-molecule imaging assays suggest that these complexes achieve distinct cellular functions by extruding large loops of DNA while they move along the chromatin fiber. In this short review, we summarize recent insights into the molecular architecture of these unconventional DNA motor complexes, their interaction with their DNA substrates, and the remarkable dynamic changes they can undergo during their ATPase reaction cycle.
摘要
结构维持染色体 (SMC) 蛋白复合物在所有生命领域的基因组三维组织中发挥着关键作用。来自染色体接触作图实验和单分子成像分析的最新见解表明，这些复合物通过在沿着染色质纤维移动时挤出 DNA 的大环来实现不同的细胞功能。在这篇简短的综述中，我们总结了这些非传统 DNA 马达复合物的分子结构、它们与 DNA 底物的相互作用以及它们在 ATP 酶反应循环中经历的显著动态变化的最新见解。
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