Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Department of Microbiology, Meiji Pharmaceutical University, Kiyose, Tokyo 204-8588, Japan.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4458-4469. doi: 10.1099/ijsem.0.004277. Epub 2020 Jul 17.
Four new yeast species belonging to the genus and two new yeast species belonging to are described for strains isolated from guano samples from bat-inhabited caves in Japan. In 2005, we reported these isolates as species based on sequence analyses of the D1/D2 domain of large subunit (LSU) rRNA genes according to available basidiomycetous yeast classification criteria; however, to date, they have not been officially published as new species with descriptions. Their phylogenetic positions have been reanalysed based on comparison of internal transcribed spacer (ITS) region sequences (including the 5.8S rRNA gene) and the D1/D2 domain of the LSU rRNA gene with those of known species; we confirmed clear separation from previously described species. Physiological and biochemical properties of the isolates also suggest their distinctiveness. Therefore, we describe (holotype JCM 12595), (JCM 12594), (JCM 12596), (JCM 12593), (JCM 12590) and (JCM 12592) as new species. showed particularly distinctive morphology including large inflated anomalous cells on the hyphae and germination from the cells, although clear clamp connections on the hyphae were not confirmed. Further study is needed to elucidate the morph of this species.
从日本蝙蝠栖息洞穴的鸟粪样本中分离出的四个属于 和两个属于 的酵母新种被描述。2005 年，我们根据现有担子菌酵母分类标准，基于大亚基（LSU）rRNA 基因的 D1/D2 结构域的序列分析，将这些分离物报告为 种；然而，迄今为止，它们尚未根据描述正式作为新种发表。根据内部转录间隔区（ITS）序列（包括 5.8S rRNA 基因）和 LSU rRNA 基因的 D1/D2 结构域与已知种的序列比较，重新分析了它们的系统发育位置；我们确认它们与先前描述的种明显分离。分离物的生理生化特性也表明它们具有独特性。因此，我们将 （模式 JCM 12595）、 （JCM 12594）、 （JCM 12596）、 （JCM 12593）、 （JCM 12590）和 （JCM 12592）描述为新种。 表现出特别独特的形态，包括菌丝上大而膨胀的异常细胞和细胞的出芽，但未确认菌丝上明显的夹状连接。需要进一步研究来阐明该种的形态。
