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长链非编码 RNA FGD5-AS1 通过海绵吸附 miR-153-3p 调控 MCL1 促进口腔癌细胞生长。

LncRNA FGD5-AS1 promotes tumor growth by regulating MCL1 via sponging miR-153-3p in oral cancer.

机构信息

Department of Stomatology Clinic, Cangzhou Central Hospital, Cangzhou, Hebei Province, China.

Department of Laboratory Medicine, Yi He Maternity Hospital, Cangzhou People's Hospital, Cangzhou, Hebei Province, China.

出版信息

Aging (Albany NY). 2020 Jul 16;12(14):14355-14364. doi: 10.18632/aging.103476.

DOI:10.18632/aging.103476
PMID:32675387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7425438/
Abstract

PURPOSE

To investigate the function of long noncoding RNA (lncRNA) FGD5-AS1 in oral cancer (OC) and to further clarify the regulation of FGD5-AS1 on miR-153-3p/MCL1 axis.

RESULTS

FGD5-AS1 was significantly increased in OC tissues and cells. Loss of FGD5-AS1 inhibited the proliferation, migration and invasion of OC cells. FGD5-AS1 acted as a sponge of miR-153-3p, and MCL1 was direct target of miR-153-3p. Forced expression of miR-153-3p or inhibition of MCL1 reversed the promoted role of FGD5-AS1 on OC cells' migration and invasion. The in vivo tumor growth assay showed that FGD5-AS1 promoted OC tumorigenesis by regulating miR-153-3p/MCL1 axis.

CONCLUSIONS

Our research revealed lncRNA FGD5-AS1 acted as an oncogene by regulating MCL1 via sponging miR-153-3p, thus providing some novel experimental basis for clinical treatment or prevention of OC.

PATIENTS AND METHODS

The mRNA expression of FGD5-AS1, miR-153-3p and MCL1 was detected by qRT-PCR. CCK8 assay, Edu assay, wound healing assay and transwell assay were used to detect the FGD5-AS1/ miR-153-3p/MCL1 axis function on proliferation, migration and invasion in OC cells. Western blot was used to calculate protein level of MCL1. Luciferase assay was used to detect the binding of miR-153-3p and MCL1, FGD5-AS1and miR-153-3p.

摘要

目的

研究长链非编码 RNA（lncRNA）FGD5-AS1 在口腔癌（OC）中的作用，并进一步阐明 FGD5-AS1 对 miR-153-3p/MCL1 轴的调控作用。

结果

在 OC 组织和细胞中，FGD5-AS1 显著上调。FGD5-AS1 的缺失抑制 OC 细胞的增殖、迁移和侵袭。FGD5-AS1 作为 miR-153-3p 的海绵，MCL1 是 miR-153-3p 的直接靶标。强制表达 miR-153-3p 或抑制 MCL1 可逆转 FGD5-AS1 对 OC 细胞迁移和侵袭的促进作用。体内肿瘤生长实验表明，FGD5-AS1 通过调节 miR-153-3p/MCL1 轴促进 OC 肿瘤发生。

结论

本研究揭示了 lncRNA FGD5-AS1 通过海绵 miR-153-3p 调节 MCL1 发挥致癌作用，为 OC 的临床治疗或预防提供了一些新的实验依据。

患者和方法

通过 qRT-PCR 检测 FGD5-AS1、miR-153-3p 和 MCL1 的 mRNA 表达。CCK8 assay、Edu assay、划痕愈合 assay 和 Transwell assay 用于检测 FGD5-AS1/miR-153-3p/MCL1 轴对 OC 细胞增殖、迁移和侵袭的作用。Western blot 用于计算 MCL1 蛋白水平。荧光素酶 assay 用于检测 miR-153-3p 和 MCL1、FGD5-AS1 和 miR-153-3p 的结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/9e2c6b58b970/aging-12-103476-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/838d563d3de6/aging-12-103476-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/b96824ba86c4/aging-12-103476-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/a4ff4fde45be/aging-12-103476-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/4349de6f39ab/aging-12-103476-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/8463f1655f15/aging-12-103476-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/09f757e1825c/aging-12-103476-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/9e2c6b58b970/aging-12-103476-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/838d563d3de6/aging-12-103476-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/b96824ba86c4/aging-12-103476-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/a4ff4fde45be/aging-12-103476-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/4349de6f39ab/aging-12-103476-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/8463f1655f15/aging-12-103476-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/09f757e1825c/aging-12-103476-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f48/7425438/9e2c6b58b970/aging-12-103476-g007.jpg

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