Department of Biomedical and Biotechnological Sciences - Section of Physiology, University of Catania, Via Santa Sofia, 97, 95123, Catania, Italy; Institute of Biomolecular Chemistry, National Research Council (C.N.R.), Via Gaifami, 18, 95026, Catania, Italy.
Institute of Biomolecular Chemistry, National Research Council (C.N.R.), Via Gaifami, 18, 95026, Catania, Italy.
Food Chem Toxicol. 2020 Oct;144:111586. doi: 10.1016/j.fct.2020.111586. Epub 2020 Jul 15.
Skin acts as a protective barrier between the body and the external environment. Skin wounds are a common inflammatory disorder for the solution of which plants and essential oils have been applied as a medical option for centuries. Origanum vulgare essential oil (OEO) is largely used in folk medicine, but its molecular mechanisms of action are not fully known. In this study, we evaluated the anti-inflammatory/antioxidant activity as well as wound healing capacity of a well-characterized OEO on human keratinocytes NCTC 2544 treated with interferon-gamma (IFN-γ) and histamine (H) or subjected to a scratch test. The expression of pro-inflammatory mediators such as reactive oxygen species (ROS), inter-cellular adhesion molecule (ICAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 were verified. The DNA damage was shown by the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG) and activation of proliferating cell nuclear antigen (PCNA). Moreover, the abnormal modification of extracellular matrix components (ECM) was examined by determining matrix metalloproteinase (MMP)-1, and -12. Compared to untreated control, OEO showed efficacy in supporting and enhancing the cell motility. In IFN-γ and H treated cells, OEO displayed a significant reduction of ROS, ICAM-1, iNOS, COX-2, 8-OHdG, MMP-1, and MMP-12. OEO proved useful to treat inflammation and support cell motility during wound healing.
皮肤是身体和外部环境之间的保护屏障。皮肤伤口是一种常见的炎症性疾病，几个世纪以来，植物和精油一直被用作一种医疗选择。牛至精油（OEO）在民间医学中被广泛应用，但它的作用机制尚不完全清楚。在这项研究中，我们评估了一种经过充分表征的 OEO 对人角质形成细胞 NCTC 2544 的抗炎/抗氧化活性以及伤口愈合能力，这些细胞用干扰素-γ（IFN-γ）和组胺（H）处理或进行划痕试验。验证了促炎介质如活性氧（ROS）、细胞间黏附分子（ICAM）-1、诱导型一氧化氮合酶（iNOS）和环氧化酶（COX）-2 的表达。通过形成 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-OHdG）和激活增殖细胞核抗原（PCNA）来显示 DNA 损伤。此外，通过测定基质金属蛋白酶（MMP）-1 和 -12 来检查细胞外基质（ECM）成分的异常修饰。与未处理的对照相比，OEO 显示出在支持和增强细胞迁移方面的功效。在 IFN-γ 和 H 处理的细胞中，OEO 显著降低了 ROS、ICAM-1、iNOS、COX-2、8-OHdG、MMP-1 和 MMP-12。OEO 被证明可用于治疗炎症并在伤口愈合过程中支持细胞迁移。
