含有丁香酚负载聚合物胶束的羟丙基纤维素水凝胶用于增强黑色素瘤治疗

Hydroxypropyl Cellulose Hydrogel Containing ssp. Essential-Oil-Loaded Polymeric Micelles for Enhanced Treatment of Melanoma.

作者信息

Kamenova Katya, Iliev Ivan, Prancheva Anna, Tuleshkov Pencho, Rusanov Krasimir, Atanassov Ivan, Petrov Petar D

机构信息

Institute of Polymers, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

出版信息

Gels. 2024 Sep 29;10(10):627. doi: 10.3390/gels10100627.

DOI:10.3390/gels10100627

PMID:39451280

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11508108/

Abstract

ssp. essential oil (OEO) is a natural oil with high therapeutic potential. For some applications, however, the development of novel formulations is still needed to improve the bioavailability and stability of OEO. In this study, we describe the fabrication of an original nanocomposite hydroxypropyl cellulose (HPC) physical hydrogel, containing OEO-loaded polymeric micelles, for topical delivery. The concentration of the main active compounds of OEO-carvacol and thymol-was determined using gas chromatography (GC) analysis. OEO was first encapsulated into Pluronic F127 micelles, and then embedded into HPC gel. Micellar and gel formulations of pure polymers and OEO-containing systems were characterized by dynamic light scattering (DLS) and rheology measurements, respectively. Selected formulations were evaluated for cytotoxicity and antiproliferative activity. The hydrogel formulation of HPC with micellar OEO (8% HPC, 2% F127, 1% OEO) exhibited sustained release of the oil and selectivity towards SH-4 tumor cells (an in vitro model of melanoma).

摘要

特种精油（OEO）是一种具有高治疗潜力的天然油。然而，对于某些应用，仍需要开发新型制剂以提高OEO的生物利用度和稳定性。在本研究中，我们描述了一种用于局部给药的新型纳米复合羟丙基纤维素（HPC）物理水凝胶的制备，该水凝胶含有负载OEO的聚合物胶束。使用气相色谱（GC）分析测定了OEO的主要活性成分——香芹酚和百里香酚的浓度。首先将OEO包封到泊洛沙姆F127胶束中，然后嵌入HPC凝胶中。分别通过动态光散射（DLS）和流变学测量对纯聚合物以及含OEO体系的胶束和凝胶制剂进行了表征。对选定的制剂进行了细胞毒性和抗增殖活性评估。含有胶束OEO的HPC水凝胶制剂（8% HPC、2% F127、1% OEO）表现出油的持续释放以及对SH - 4肿瘤细胞（黑色素瘤的体外模型）的选择性。