Arsyad Aryadi, Idris Irfan, Rasyid Andi A, Usman Rezky A, Faradillah Kiki R, Latif Wa Ode U, Lubis Zidni I, Aminuddin Aminuddin, Yustisia Ika, Djabir Yulia Y
Department of Physiology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Biomedical Science Study Program, Postgraduate School, Hasanuddin University, Makassar, Indonesia.
J Nutr Metab. 2020 Jun 19;2020:3642035. doi: 10.1155/2020/3642035. eCollection 2020.
Ketogenic diet has been used as supportive therapy in a range of conditions including epilepsy, diabetes mellitus, and cancer.
This study aimed to investigate the effects of long-term consumption of ketogenic diet on blood gas, hematological profiles, organ functions, and superoxide dismutase level in a rat model.
Fifteen male Wistar rats were divided into control ( = 8) and ketogenic ( = 7) groups. Controls received standard diet contained 52.20% of carbohydrates, 7.00% fat, and 15.25% protein; meanwhile, the ketogenic group received a high-fat-low-carbohydrate diet which contained 5.66% of carbohydrate, 86.19% fat, and 8.15% protein. All rats were caged individually and received 30g of either standard or high-fat-low-carbohydrate pellets. The experiment was carried out for 60 days before the blood samples were taken and analyzed to obtain blood gas, cell counts, organ biomarkers, and plasma antioxidant superoxide dismutase (SOD) levels.
The rats subjected to ketogenic diet experienced a marked decrease in body weight, blood sugar, and increased blood ketones ( < 0.05). The average blood pH was 7.36 ± 0.02 and base excess was -5.57 ± 2.39 mOsm/L, which were significantly lower than controls ( < 0.05). Hematological analysis showed significantly lower erythrocyte, hemoglobin, and hematocrit levels. No significant changes were found in alanine aminotransferase, aspartate aminotransferase, urea, and creatinine levels, indicating normal liver and kidney functions. Nevertheless, plasma SOD level significantly reduced with ketogenic diet.
Long-term ketogenic diet induces metabolic acidosis, anemia, and reduced antioxidant enzyme level in rats following 60 days of consuming high-fat-low-carbohydrate diet.
生酮饮食已被用作包括癫痫、糖尿病和癌症在内的一系列病症的支持性疗法。
本研究旨在调查长期食用生酮饮食对大鼠模型的血气、血液学指标、器官功能和超氧化物歧化酶水平的影响。
将15只雄性Wistar大鼠分为对照组（n = 8）和生酮组（n = 7）。对照组给予标准饮食，其中碳水化合物含量为52.20%，脂肪含量为7.00%，蛋白质含量为15.25%；同时，生酮组给予高脂肪低碳水化合物饮食，其中碳水化合物含量为5.66%，脂肪含量为86.19%，蛋白质含量为8.15%。所有大鼠单独饲养，每天给予30克标准或高脂肪低碳水化合物颗粒饲料。实验持续60天，之后采集血样并进行分析，以获取血气、细胞计数、器官生物标志物和血浆抗氧化超氧化物歧化酶（SOD）水平。
接受生酮饮食的大鼠体重、血糖显著下降，血酮升高（P < 0.05）。平均血pH值为7.36 ± 0.02，碱剩余为 -5.57 ± 2.39 mOsm/L，均显著低于对照组（P < 0.05）。血液学分析显示红细胞、血红蛋白和血细胞比容水平显著降低。丙氨酸转氨酶、天冬氨酸转氨酶、尿素和肌酐水平无显著变化，表明肝肾功能正常。然而，生酮饮食使血浆SOD水平显著降低。
长期食用高脂肪低碳水化合物饮食60天后，生酮饮食会导致大鼠代谢性酸中毒、贫血和抗氧化酶水平降低。
