State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, People's Republic of China.
School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.
Emerg Microbes Infect. 2020 Dec;9(1):1793-1803. doi: 10.1080/22221751.2020.1797542.
Lethal infection of wild birds with different subtypes of H5 viruses continuously occur. To investigate the genetic evolution and pathogenicity of H5 viruses in wild birds, we performed a detailed genetic and biologic analysis of 27 viruses, including H5N1, H5N2, H5N6, and H5N8 subtypes, that were responsible for avian influenza outbreaks in wild birds in China over the past decade. We found that these 27 viruses, bearing different clades/subclades of HA, were complicated reassortants and formed 12 different genotypes. Ten of the viruses tested were highly pathogenic in chickens, but showed distinct pathotypes in ducks and mice. Five of these 10 viruses, which were all from clade2.3.4.4, could bind human-type receptors. Our findings reveal the diversity of the genetic and biologic properties of H5 viruses circulating in wild birds and highlight the need to carefully monitor and evaluate the risks these viruses pose to animal and public health.
不同亚型 H5 病毒对野生鸟类的致死性感染持续发生。为了研究野生鸟类中 H5 病毒的遗传进化和致病性，我们对过去十年在中国野生鸟类中引发禽流感的 27 种病毒（包括 H5N1、H5N2、H5N6 和 H5N8 亚型）进行了详细的遗传和生物学分析。我们发现，这些携带不同 HA 分支/亚分支的 27 种病毒是复杂的重组体，并形成了 12 种不同的基因型。其中 10 种测试病毒对鸡具有高致病性，但在鸭和鼠中表现出不同的病型。这 10 种病毒中的 5 种都来自 2.3.4.4 分支，能够结合人源受体。我们的研究结果揭示了在野生鸟类中流行的 H5 病毒的遗传和生物学特性的多样性，并强调需要仔细监测和评估这些病毒对动物和公共卫生构成的风险。
