Jang Hyungjun, Oh Jusung, Ki Hangil, Kim Min-Gon
Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea.
Analyst. 2020 Aug 24;145(17):5740-5743. doi: 10.1039/d0an00905a.
The monosaccharide 1,5-anhydroglucitol (1,5-AG) is a known indicator of glucose levels. Conventional 1,5-AG quantification methods with enzyme-based sensors using pyranose oxidase (PROD) require elimination of interference from the sample (a laborious and time-consuming process), as PROD cannot distinguish 1,5-AG from other sugars. We developed a one-step paper-based sensor for detecting 1,5-AG using glucose oxidase, catalase, and mutarotase that eliminates excess glucose, which interferes with 1,5-AG detection. This sensor consists of two compartments for the quantification of glucose and 1,5-AG and reflects the concentration of these targets after reaction with water or spiked human urine. The limit of detection of the sensor was 0.9 mg dL-1 for glucose and 3.2 μg mL-1 for 1,5-AG.
单糖1,5-脱水葡萄糖醇（1,5-AG）是一种已知的葡萄糖水平指标。使用吡喃糖氧化酶（PROD）的基于酶的传感器进行传统的1,5-AG定量方法需要消除样品中的干扰（这是一个费力且耗时的过程），因为PROD无法区分1,5-AG与其他糖类。我们开发了一种使用葡萄糖氧化酶、过氧化氢酶和变旋酶检测1,5-AG的一步式纸质传感器，该传感器可消除干扰1,5-AG检测的过量葡萄糖。该传感器由两个隔室组成，用于定量葡萄糖和1,5-AG，并在与水或加标的人尿反应后反映这些目标物的浓度。该传感器对葡萄糖的检测限为0.9 mg dL-1，对1,5-AG的检测限为3.2 μg mL-1。
