从高等植物叶片中提取光合色素的溶剂体系比较

Comparison of solvent regimes for the extraction of photosynthetic pigments from leaves of higher plants.

作者信息

Dunn Jodie L, Turnbull Johanna D, Robinson Sharon A

机构信息

Institute for Conservation Biology, Department of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia.

Institute for Conservation Biology, Department of Biological Sciences, University of Wollongong, Wollongong, NSW 2522, Australia. Corresponding author; email:

出版信息

Funct Plant Biol. 2004 Mar;31(2):195-202. doi: 10.1071/FP03162.

DOI:10.1071/FP03162

PMID:32688891

Abstract

The relative efficiency of methanol- and acetone-based solvents for the extraction of pigments from photosynthetic tissues of plant was compared, together with the advantages of multiple versus single extractions. The two commonly employed triple acetone extractions (100 : 80 : 80% and 85 : 100 : 100%) performed comparably for most pigments and for all plant species tested. Single extractions with either 96% methanol or 85% acetone failed to extract the more hydrophobic pigments, especially β-carotene. We conclude that multiple extractions that combine pure and aqueous (80-85%) acetone are preferable for extraction of the full range of pigments. These results suggest that previous studies that have utilised aqueous methanol (especially in a single extraction) have probably underestimated the concentration of β-carotene relative to other pigments.

摘要

比较了基于甲醇和丙酮的溶剂从植物光合组织中提取色素的相对效率，以及多次提取与单次提取的优势。两种常用的三次丙酮提取法（100 : 80 : 80%和85 : 100 : 100%）对大多数色素以及所有测试植物物种的提取效果相当。用96%甲醇或85%丙酮进行单次提取无法提取出疏水性更强的色素，尤其是β-胡萝卜素。我们得出结论，将纯丙酮和水相（80 - 85%）丙酮结合的多次提取法更适合提取全范围的色素。这些结果表明，以往使用水相甲醇（尤其是单次提取）的研究可能低估了β-胡萝卜素相对于其他色素的浓度。