Silveira Carolina Hahn da, Vieceli Viviane, Clerici Dariane Jornada, Santos Roberto Christ Vianna, Iglesias Bernardo Almeida
Laboratório De Bioinorgânica e Materiais Porfirínicos, Departamento De Química, Universidade Federal De Santa Maria, UFSM, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil.
Laboratório De Pesquisa Em Microbiologia Oral, Departamento De Microbiologia e Parasitologia, Universidade Federal De Santa Maria, Av. Roraima 1000, 97105-900, Santa Maria, RS, Brazil.
Photodiagnosis Photodyn Ther. 2020 Sep;31:101920. doi: 10.1016/j.pdpdt.2020.101920. Epub 2020 Jul 18.
In this work, we report the antimicrobial photoinactivation using tetra-cationic porphyrins with peripheral palladium(II) complexes against bacterial strains. Two different isomeric Pd(II)-porphyrins were used and applied in PDI experiments in the strains Gram negative bacilli Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and a Gram positive cocci Staphylococcus aureus. All experiments are conducted with adequate concentration (without aggregation) of photosensitizer under white-light irradiation over 2 h . We showed that the most effective PS caused a significative reduction in the concentration of viable bacteria. The present results show that porphyrin with positively charge at meta position (3-PtTPyP) are more efficient photosensitizer against bacteria strains. In this way, tetra-cationic porphyrins might be promising antibacterial PDI agents with potential applications in clinical cases.
在本研究中，我们报道了使用带有外周钯（II）配合物的四阳离子卟啉对细菌菌株进行抗菌光灭活。使用了两种不同的异构钯（II）-卟啉，并将其应用于革兰氏阴性杆菌大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌以及革兰氏阳性球菌金黄色葡萄球菌的光动力灭活（PDI）实验中。所有实验均在白光照射2小时以上的条件下，使用适当浓度（无聚集）的光敏剂进行。我们发现，最有效的光敏剂导致活菌浓度显著降低。目前的结果表明，间位带正电荷的卟啉（3-PtTPyP）对细菌菌株是更有效的光敏剂。通过这种方式，四阳离子卟啉可能是有前景的抗菌光动力灭活剂，在临床病例中具有潜在应用价值。
