胰岛素样生长因子1诱导的人脐带华通氏胶间充质干细胞条件培养基（IGF1-hWJMSCs-CM）对骨关节炎的影响
Effect of Conditioned Medium from IGF1-Induced Human Wharton's Jelly Mesenchymal Stem Cells (IGF1-hWJMSCs-CM) on Osteoarthritis.
作者信息
Kusuma Hanna Sari Widya, Widowati Wahyu, Gunanegara Rimonta Febby, Juliandi Berry, Lister Nyoman Ehrich, Arumwardana Seila, Yusepany Dewani Tediana, Artie Dwi Surya, Nataya Enden Dea, Gunawan Kamila Yashfa, Sholihah Ika Adhani, Girsang Ermi, Ginting Chrismis Novalinda, Bachtiar Indra, Murti Harry
机构信息
Biomolecular and Biomedical Research Center, Aretha Medika, Utama, Bandung, West Java, Indonesia.
Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
出版信息
Avicenna J Med Biotechnol. 2020 Jul-Sep;12(3):172-178.
BACKGROUND
Osteoarthritis (OA) is a chronic disease that attacks joints and bones which can be caused by trauma or other joint diseases. Stem cell and Conditioned Medium (CM) of stem cells are developed for OA therapy, which is minimally invasive. It can decrease inflammation and be a replacement for knee surgery. This study aimed to utilize human Wharton's Jelly-Mesenchymal Stem Cells (hWJMSCs) as an alternative OA therapy.
METHODS
CM from hWJMSCs induced by IGF1 was collected. The OA cells model (IL1β-CHON002) culture was treated as follows: 1) with hWJMSCs-CM 15% (v/v); 2) with hWJMSCs-CM 30% (v/v); 3) with IGF1-hWJMSCs (IGF1-hWJMSCs-CM) 15% (v/v); 4) with IGF1-hWJMSCs-CM 30% (v/v). Parameters including inflammatory cytokines (IL10 and TNFα), extracellular matrix degradation (MMP3 expression), and chondrogenic marker ( expression) were determined.
RESULTS
The most significant increase in chondrogenic markers was found in IL1β-CHON002 treatment using 15% CM of hWJMSCs induced with IGF1. CM of hWJMSCs can reduce inflammatory cytokines (TNFα and IL10) and matrix degradation mediator MMP3. Better result was gained from IGF1-induced hWJMSCs-CM.
CONCLUSION
CM of IGF1-hWJMSCs reduce inflammation while repairing injured joint in the human chondrocyte OA model.
背景
骨关节炎（OA）是一种侵袭关节和骨骼的慢性疾病，可由创伤或其他关节疾病引起。干细胞和干细胞条件培养基（CM）被开发用于OA治疗，这种治疗微创，可减轻炎症，有望替代膝关节手术。本研究旨在利用人脐带华通氏胶间充质干细胞（hWJMSCs）作为OA的替代治疗方法。
方法
收集由IGF1诱导的hWJMSCs的CM。OA细胞模型（IL1β-CHON002）培养物按以下方式处理：1）用15%（v/v）的hWJMSCs-CM；2）用30%（v/v）的hWJMSCs-CM；3）用15%（v/v）的IGF1-hWJMSCs（IGF1-hWJMSCs-CM）；4）用30%（v/v）的IGF1-hWJMSCs-CM。测定包括炎性细胞因子（IL10和TNFα）、细胞外基质降解（MMP3表达）和成软骨标志物（表达）等参数。
结果
在用IGF1诱导的hWJMSCs的15% CM处理IL1β-CHON002时，成软骨标志物的增加最为显著。hWJMSCs的CM可降低炎性细胞因子（TNFα和IL10）和基质降解介质MMP3。IGF1诱导的hWJMSCs-CM取得了更好的效果。
结论
IGF1-hWJMSCs的CM在修复人类软骨细胞OA模型中受损关节的同时减轻炎症。
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