Ma Jinhu, Feng Xinxin, Yang Xiaohuan, Cao Yongheng, Zhao Weifeng, Sun Liangliang
College of Engineering, Shanxi Agricultural University, Taigu, 030801, China.
College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China.
Plant Divers. 2020 Mar 29;42(3):174-180. doi: 10.1016/j.pld.2020.02.001. eCollection 2020 Jun.
The extract of crofton weed () inhibits seed germination and weed growth; however, the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear. In this study, we investigated the effects of the leaf extract of crofton weed (LECW) on primary root (PR) growth in maize seedlings. Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner. Physiological analysis indicated that the LECW induced reactive oxygen species (ROS) accumulation in root tips, thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips, finally leading to cell death in root border cells (RBCs) and PR growth inhibition. The LECW also inhibited pectin methyl esterase (PME) activity, thereby decreasing the RBC number. Taken together, our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in RBCs. The present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants.
飞机草提取物抑制种子萌发和杂草生长；然而，飞机草提取物对幼苗生长和根系发育调节作用的生理机制仍不清楚。在本研究中，我们研究了飞机草叶提取物（LECW）对玉米幼苗初生根（PR）生长的影响。用LECW处理以剂量依赖的方式显著抑制种子萌发和幼苗生长。生理分析表明，LECW诱导根尖活性氧（ROS）积累，从而导致根尖根冠和伸长区细胞肿胀和变形，最终导致根边缘细胞（RBCs）死亡和PR生长受抑制。LECW还抑制果胶甲酯酶（PME）活性，从而减少RBCs数量。综上所述，我们的结果表明，LECW通过诱导ROS积累和随后RBCs细胞死亡来抑制PR生长。本研究有助于更好地理解LECW如何改变根系发育，并为评估飞机草提取物对植物的毒性提供见解。
