University of Belgrade - Vinča Institute of Nuclear Sciences - National Institute of Republic of Serbia, Mike Petrovića Alasa 12, Belgrade, Serbia.
Department of Microbiology, University of Belgrade - Faculty of Biology, Studentski trg 16, Belgrade, Serbia.
Arch Oral Biol. 2020 Sep;117:104842. doi: 10.1016/j.archoralbio.2020.104842. Epub 2020 Jul 16.
The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth.
The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA.
The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of logCFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of logCFU was 1.69 and 1.14, respectively).
Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
本研究旨在评估香茅和香桃木精油的抗生物膜效果，并评估油基根管冲洗剂对从儿童患者根管中分离出的、在牙本质小管内形成的多菌种生物膜的破坏潜能。
采用 GC-MS 对精油进行特征分析，通过微量稀释和 MTT 测定法检测其抗菌和细胞毒性。测定冲洗剂的颗粒大小和多分散指数。采用 MALDI-TOF 对小儿患者根管内分离的细菌进行鉴定。使用变形链球菌、血链球菌和粪肠球菌构建多菌种生物膜。通过结晶紫和平板计数法测定精油和冲洗剂的抗生物膜特性。采用单因素方差分析进行统计学分析。
生物膜生物量筛选结果显示香茅精油具有破坏生物膜的潜力。平板计数法显示两种精油均能有效降低细胞活力：在生物膜中（减少的 logCFU 为 2.75-2.87）和浮游生物中（减少的 logCFU 为 2.75-2.87）效果显著。香茅精油基冲洗剂对生物膜的活性与 1.5%次氯酸钠相当，对香桃木精油的活性则几乎高 2 倍。与对照组（logCFU 减少 1.69 和 1.14）相比，1.5%次氯酸钠、生理盐水和油基冲洗剂的连续冲洗对香茅精油的效果更好。
香茅精油和油基冲洗剂针对变形链球菌、血链球菌和粪肠球菌生物膜具有显著的活性，有望进一步推动牙髓病学的研究。
