建立一种快速准确的 UHPLC-PDA-FL 方法，用于葡萄中酚类化合物的定量分析。
Development of a rapid and accurate UHPLC-PDA-FL method for the quantification of phenolic compounds in grapes.
机构信息
Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Agrifood Campus of International Excellence (ceiA3), IVAGRO, P.O. Box 40, 11510 Puerto Real, Cadiz, Spain.
Centro IFAPA Rancho de la Merced, Carretera de Trebujena, km 3.2, Apdo. 589, 11471 Jerez de la Frontera, Cadiz, Spain.
出版信息
Food Chem. 2021 Jan 1;334:127569. doi: 10.1016/j.foodchem.2020.127569. Epub 2020 Jul 18.
Grapes are a great source of phenolic compounds, which have excellent antioxidant properties. Efficient analytical methods are necessary to selectively and precisely determine these compounds content in grapes. In this study, a reverse-phase ultra-high performance liquid chromatography (UHPLC) method with fluorescence and photodiode array detection has been developed to determine and quantify 27 of the main phenolic compounds present in grapes. An ACQUITY UPLC® BEH C18 (50 mm × 2.1 mm i.d., 1.7 mm particle size) column was employed. A gradient method was developed and column temperature (25-55 °C), as well as flow rate (0.6-0.75 mL min), were optimized. The optimum conditions allowed the separation of all the compounds in less than 9 min. The method was validated and demonstrated excellent detection and quantification limits, precision, and selectivity. Finally, several grape varieties were studied in order to demonstrate the applicability of the method to the analysis of real matrix samples.
葡萄是酚类化合物的重要来源，具有出色的抗氧化性能。需要有效的分析方法来选择性和精确地测定葡萄中这些化合物的含量。在这项研究中，开发了一种反相超高效液相色谱（UHPLC）方法，结合荧光和光电二极管阵列检测，用于测定和定量葡萄中存在的 27 种主要酚类化合物。使用了 ACQUITY UPLC® BEH C18（50mm×2.1mm id，1.7mm 粒径）柱。开发了梯度方法，并优化了柱温（25-55°C）和流速（0.6-0.75mLmin）。最佳条件允许在不到 9 分钟的时间内分离所有化合物。该方法经过验证，具有出色的检测和定量限、精密度和选择性。最后，研究了几种葡萄品种，以证明该方法适用于分析实际基质样品。
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