Carrera Ceferino, Aliaño-González María José, Valaityte Monika, Ferreiro-González Marta, Barbero Gerardo F, Palma Miguel
Department of Analytical Chemistry, Faculty of Sciences, Agrifood Campus of International Excellence (ceiA3), IVAGRO, University of Cadiz, 11510 Puerto Real, Spain.
Department of Biology, Faculty of Marine and Environmental Sciences, University of Cadiz, 11510 Puerto Real, Spain.
Antioxidants (Basel). 2021 Aug 28;10(9):1375. doi: 10.3390/antiox10091375.
Purple potato is one of the least known and consumed potato varieties. It is as rich in nutrients, amino acids and starches as the rest of the potato varieties, but it also exhibits a high content of anthocyanins, which confer it with some attractive health-related properties, such as antioxidant, pain-relieving, anti-inflammatory and other promising properties regarding the treatment of certain diseases. A novel methodology based on ultrasound-assisted extraction has been optimized to achieve greater yields of anthocyanins. Optimal extraction values have been established at 70 °C using 20 mL of a 60% MeOH:HO solution, with a pH of 2.90 and a 0.5 s cycle length at 70% of the maximum amplitude for 15 min. The repeatability and intermediate precision of the extraction method have been proven by its relative standard deviation (RSD) below 5%. The method has been tested on Vitelotte, Double Fun, Highland and Violet Queen potatoes and has demonstrated its suitability for the extraction and quantification of the anthocyanins found in these potato varieties, which exhibit notable content differences. Finally, the antioxidant capacity of these potato varieties has been determined by means of 2,2-diphenyl-1-picrylhydrazyl (DDPH) radical scavenging and the values obtained were similar to those previously reported in the literature.
紫薯是最鲜为人知且食用较少的马铃薯品种之一。它与其他马铃薯品种一样富含营养、氨基酸和淀粉，但同时还含有高含量的花青素，赋予其一些吸引人的与健康相关的特性，如抗氧化、止痛、抗炎以及在某些疾病治疗方面的其他潜在特性。一种基于超声辅助提取的新方法已得到优化，以实现更高的花青素产量。已确定最佳提取条件为：在70℃下使用20 mL 60%甲醇:水的溶液，pH值为2.90，以最大振幅的70%进行0.5 s的循环，持续15分钟。该提取方法的重复性和中间精密度已通过其相对标准偏差（RSD）低于5%得到证明。该方法已在维特洛特、双福、高地和紫皇后马铃薯上进行测试，并证明其适用于提取和定量这些马铃薯品种中所含的花青素，这些品种的花青素含量存在显著差异。最后，通过2,2-二苯基-1-苦基肼（DPPH）自由基清除法测定了这些马铃薯品种的抗氧化能力，所得值与文献中先前报道的值相似。
